| Автор | NARUSE, Hidenori |
| Автор | SAKAI, Hikoichi |
| Дата выпуска | 1981 |
| dc.description | A new factor that not only inhibits brain microtubule assembly but also causes depolymerization of pre-assembled microtubules was purified from unfertilized sea urchin egg cortices. The purified fraction contained neither proteins nor nucleic acids. The inhibitory activity of the fraction was heat-stable and insensitive to various proteases, but decreased on treatment with glycosidases or with periodic acid. The purified factor was identified as a polysaccharide by analysis of the sugar composition. The sedimentation constant of the factor was estimated to be 9S by sucrose density gradient centrifugation. Polyacrylamide gel electrophoresis of the factor revealed a single broad band stained with periodic acid-Schiff reagent. The factor completely inhibited microtubule assembly at a factor: microtubule protein ratio of 0.03 (w/w). The polysaccharide was found to bind to microtubule-associated proteins, thereby inhibiting microtubule assembly. |
| Формат | application.pdf |
| Издатель | Oxford University Press |
| Копирайт | © 1981, by the Japanese Biochemical Society |
| Тема | Articles |
| Название | Evidence that a Polysaccharide from the Cortex of Sea Urchin Egg Inhibits Microtubule Assembly through Its Binding to Microtubule-Associated Proteins |
| Тип | research-article |
| Electronic ISSN | 1756-2651 |
| Print ISSN | 0021-924X |
| Журнал | Journal of Biochemistry |
| Том | 90 |
| Первая страница | 581 |
| Последняя страница | 587 |
| Аффилиация | Department of Biophysics and Biochemistry, Faculty of Science, The University of TokyoHongo, Bunkyo-ku, Tokyo 113 |
| Выпуск | 3 |
