Автор |
Keshet (Baksht), Eli |
Автор |
Gal, Alma |
Автор |
Groot, Nathan de |
Автор |
Hochberg, Abraham A. |
Автор |
Sprinzl, Mathias |
Автор |
Cramer, Friedrich |
Дата выпуска |
1977 |
dc.description |
Phe-tRNA<sup>phe</sup> from yeast containing 2-thiocytidine or 5-iodocytidine in position 75 of the polynucleotide chain or Phe-tRNA<sup>phe</sup> in which both positions 74 and 75 were substituted by 5-lodocytidine were investigated in the poly U-dependent polyphenylalanine synthesis on ribosomes from rabbit reticulocytes. Phe-tRNA<sup>phe</sup> Cps<sup>2</sup>CpA was nearly as active as the native Phe-tRNA<sup>phe</sup>-CpCpA in the overall process. Phe-tRNA<sup>Phe</sup>-Cpi<sup>5</sup>CpA as well as Phe-tRNA<sup>Phe</sup>-i<sup>5</sup>Cpi<sup>5</sup>CpA were considerably less active than the native species. In vestigation of individual steps of protein biosynthesis with these modified substrates revealed that the donor activity of peptidyl-tRNAs which contain 5-iodocytidine in their 3′-terminus is strongly impaired suggesting exacting structural requirements for the interaction of the C<sup>p</sup>C<sup>p</sup>A end of tRNA with the ribosomal P-site. |
Формат |
application.pdf |
Издатель |
Oxford University Press |
Копирайт |
© 1977 Information Retrieval Limited |
Тема |
Articles |
Название |
Properties of phenylalanine transfer ribonucleic acid with modified 3′-terminal end in protein biosynthesis using a rabbit reticulocyte cell-free system: effect of the replacement of cytidine residues from the CpCpA end of tRNA by 5-iodocytidine or 2-thiocytidine |
Тип |
research-article |
Electronic ISSN |
1362-4962 |
Print ISSN |
0305-1048 |
Журнал |
Nucleic Acids Research |
Том |
4 |
Первая страница |
2205 |
Последняя страница |
2212 |
Аффилиация |
Department of Biological Chemistry, The Hebrew University of JerusalemIsrael |
Аффилиация |
Sprinzl Mathias; Abteilung Chemie, Max-Planck-Institut für experimentelle Medizin |
Аффилиация |
Cramer Friedrich; Abteilung Chemie, Max-Planck-Institut für experimentelle Medizin |
Выпуск |
7 |