| Автор | Nakajo, Shigeo |
| Автор | Theerasilp, Sarroch |
| Автор | Nakaya, Kazuyasu |
| Автор | Nakamura, Yasuharu |
| Автор | Kurihara, Yoshie |
| Дата выпуска | 1988 |
| dc.description | We have developed an enzyme-linked immunoabsorbent assay for miraculin, a glycoprotein which is capable of modifying sour taste into sweet. Antiserum against purified miraculin was raised in rabbits and the anti-miraculin in IgG was purified using a Protein A-Cellulofine column and then conjugated with horseradish peroxidase. The established enzyme immunoassay method was able to accurately quantitate pg amounts of miraculin in crude extracts of miracle fruit. The amounts of miraculin in miracle fruit increased dramatically seven weeks after pollination and at eight weeks reached 102 μg/mg protein in the fruit. |
| Формат | application.pdf |
| Издатель | Oxford University Press |
| Копирайт | © IRL Press Ltd. |
| Тема | ORIGINAL ARTICLES |
| Название | A quantitative enzyme immunoassay for miraculin in Richadella dulcifica (miracle fruit) |
| Тип | other |
| Electronic ISSN | 1464-3553 |
| Print ISSN | 0379-864X |
| Журнал | Chemical Senses |
| Том | 13 |
| Первая страница | 663 |
| Последняя страница | 669 |
| Аффилиация | Nakajo Shigeo; Laboratory of Biological Chemistry, School of Pharmaceutical Sciences, Showa University |
| Аффилиация | Theerasilp Sarroch; Department of Chemistry, Faculty of Education, Yokohama National University |
| Аффилиация | Nakaya Kazuyasu; Laboratory of Biological Chemistry, School of Pharmaceutical Sciences, Showa University |
| Аффилиация | Nakamura Yasuharu; Laboratory of Biological Chemistry, School of Pharmaceutical Sciences, Showa University |
| Аффилиация | Kurihara Yoshie; Department of Chemistry, Faculty of Education, Yokohama National University |
| Выпуск | 4 |
