| Автор | Daum, Robert S. |
| Автор | Gupta, Sangeeta |
| Автор | Sabbagh, Reem |
| Автор | Milewski, Wieslawa M. |
| Дата выпуска | 1992 |
| dc.description | “Derivative isolates” with 4- to 8-fold and 8- to 16-fold increases in MICs of vancomycin and teicoplanin, respectively, were selected from 2 susceptible clinical isolates of Staphylococcus aureus by serial incubation in low-level vancomycin. A protein of ∼39 kDa was demonstrable in the cytoplasmic fraction and occasionally in the membrane fraction by SDS-PAGE of both derivatives. This protein was purified by DEAE chromatography, preparative SDS-PAGE, and electroelution. Derivative bacteria were larger on transmission electron microscopy, had thicker cell walls, and had changes in colony morphology on solid media. Further evidence for cell wall reorganization included loss of phage and capsular typing, decreased susceptibility to lysostaphin/lysozyme killing, and changes in condition for detection of optimal coagulase activity. The mechanism of decreased susceptibility to glycopeptide antibiotics among S. aureus derivative isolates is uncertain. The production of the ∼39-kDa cytoplasmic protein and cell wall reorganization may mediate changed affinity of glycopeptide-peptidoglycan binding or impairment of glycopeptide access to its cell wall target. |
| Формат | application.pdf |
| Издатель | The University of Chicago Press |
| Копирайт | © 1992 The University of Chicago |
| Тема | Major Articles |
| Название | Characterization of <sub>Staphylococcus aureus</sub> Isolates with Decreased Susceptibility to Vancomycin and Teicoplanin: Isolation and Purification of a Constitutively Produced Protein Associated with Decreased Susceptibility |
| Тип | research-article |
| DOI | 10.1093/infdis/166.5.1066 |
| Electronic ISSN | 1537-6613 |
| Print ISSN | 0022-1899 |
| Журнал | The Journal of Infectious Diseases |
| Том | 166 |
| Первая страница | 1066 |
| Последняя страница | 1072 |
| Аффилиация | Wyler Children's Hospital and Department of Pediatrics, University of Chicago, Illinois |
| Выпуск | 5 |
