Cambridge University Press по журналам "Zygote"
Отображаемые элементы 1-20 из 411
-
(Cambridge University Press. Cambridge, UK, 1993-08-01)
-
(Cambridge University Press. Cambridge, UK, 1995-11-01)The intracellular pH of the mouse egg was measured during fertilisation to determine whether an increase in pH accompanies activation of this mammalian egg. The pH-sensitive dye BCECF [2′,7′-bis-(2-carboxyethyl)-5(and-6) ...
-
(Cambridge University Press. Cambridge, UK, 2011-11-01)We investigated whether the small litter size in the 129 inbred mouse strain results from a reduction in oocyte fertilizability. Sensitivity of the zona pellucida to α-chymotrypsin was examined for oocytes collected at 14 ...
-
(Cambridge University Press. Cambridge, UK, 2009-11-01)The identification of early changes in the sperm plasmalemma is currently a factor in the improvement of freezing protocols. We analysed the presence of active caspases in freeze–thawed (FT) dog spermatozoa, and evaluated ...
-
(Cambridge University Press. Cambridge, UK, 1993-05-01)Spermatozoa from the mollusc Ostrea edulis are capable of fusing to and entering de-chorionated ascidian eggs. During interaction they generate activation currents, comparable to the fertilisation currents induced by ...
-
(Cambridge University Press. Cambridge, UK, 1995-08-01)We have used confocal microscopy and a fluorescent calmodulin probe to examine the mechanism of localisation of calmodulin during the first cell cycle of the sea urchin zygote. Using fluoresceincalmodulin, calmodulin can ...
-
(Cambridge University Press. Cambridge, UK, 2011-05-01)The adenosine monophosphate-activated protein kinase (AMPK) activators, 5′-aminoimidazole-4-carboxamide 1-β-d-ribofuranoside (AICAR) and metformin (MET), inhibit resumption of meiosis in bovine cumulus-enclosed oocytes ...
-
(Cambridge University Press. Cambridge, UK, 1994-11-01)The present study was designed to examine the ability of bovine oocytes, after germinal vesicle breakdown (GVBD), to be activated by sperm penetration and the sequence of sperm nuclear transformation. Bovine oovytes cultured ...
-
(Cambridge University Press. Cambridge, UK, 1995-08-01)To investigate the involvement of mitogen-activated protein kinase(MAP kinase) in meiotic maturation of porcine oocytes, we assayed MAP kinase activity using basic protein(MBP) as a substrate. MAP kinase activity was low ...
-
(Cambridge University Press. Cambridge, UK, 1997-11-01)We have studied the developmental activation of the metaphase checkpoint, and the consequences of activating this checkpoint, in the zebrafish embryo. (1) Treatment with nocodazole (a microtubule destabiliser) before ...
-
(Cambridge University Press. Cambridge, UK, 2009-11-01)We have investigated whether culture in glycine-containing medium affects subsequent glycine transport by the specific transport system, GLYT1, which is the sole glycine transporter in fertilized mouse ova. When fertilized ...
-
(Cambridge University Press. Cambridge, UK, 2009-08-01)In this study, we examined a modified cryoloop vitrification protocol in the cryopreservation of mature mouse oocytes. The mature mouse oocytes were first vitrified and then warmed up in a modified cryoloop vitrification ...
-
(Cambridge University Press. Cambridge, UK, 1993-08-01)Localised, maternally synthesised RNAs and proteins play an important role in an early animal embryogenesis. In Drosophila, genetic screens have recovered a number of maternal effect loci that encode localised products in ...
-
(Cambridge University Press. Cambridge, UK, 2009-11-01)Bufo arenarum oocytes are oviposited surrounded by jelly coats, one component of the extracellular matrix required for fertilization. The secretion, released to the oviductal lumen, was analysed by SDS-PAGE. The coomassie ...
-
(Cambridge University Press. Cambridge, UK, 1993-08-01)An erythrochyte anti-ankryin antibody was used to investigate the presence and distribution of ankryin in eggs and embryos of ascidian species with different modes of development. In eggs of the indirect developer Ascidia ...
-
(Cambridge University Press. Cambridge, UK, 2010-11-01)The overnight preservation of bovine ovaries would be highly useful in the subsequent harvest of viable oocytes for reproductive study. The present study aimed to optimize conditions for overnight preservation of bovine ...
-
(Cambridge University Press. Cambridge, UK, 1996-11-01)This paper addresses the proposition, first advanced by Wilson (1925), that successful embryogenesis depends on an ordered series of events in oogenesis. It is at the completion of this varied set of intracellular changes ...
-
(Cambridge University Press. Cambridge, UK, 2009-08-01)The present study evaluates the role of apoptotic cell death and DNA methylation reprogramming in early developmental failures occurring in embryos at the 2-cell stage. Mouse 2-cell embryos were cultured in vitro and treated ...
-
(Cambridge University Press. Cambridge, UK, 1993-11-01)Sand dollar eggs were microinjected with botulinum C3 exoenzyme, an ADP-ribosyltransferase from Clostridium botulinum that specifically ADP-ribosylates and inactivates rho proteins. C3 exoenzyme microinjected during nuclear ...
-
(Cambridge University Press. Cambridge, UK, 2007-08-01)Though much is known about various aspects of reproductive biology of amphibia, there is little information on the cellular and mechanistic basis of assembly of ovarian follicles in this group. This is especially true of ...
Отображаемые элементы 1-20 из 411