Автор |
Pourreau-Schneider, Natalie |
Дата выпуска |
1975 |
dc.description |
SummaryPropagation of the transplantable acute rat leukemia L5222 in vitro was highly dependent on the reducing agent 2-mercaptoethanol (2-Me). Maximum growth promotion was obtained in the simultaneous presence of 1.25–2.5×10<sup>−4</sup> m 2-Me and 30% fetal bovine serum in enriched tissue culture medium NCTC 135. A short exposure to the reducing agent insured growth of the leukemia cells for 5 days, but extended culture necessitated constant presence of the thiol. After 2–8 days of culture, inoculation of the cell suspension produced widespread leukemia in inbred BD IX rats. |
Формат |
application.pdf |
Издатель |
Oxford University Press |
Тема |
Clinical and Laboratory Investigations on Animals |
Название |
Brief Communication: In Vitro Growth Promotion of Rat Leukemia L5222 Cells in the Presence of 2-MercaptoethanoI2 |
Тип |
research-article |
DOI |
10.1093/jnci/55.6.1467 |
Electronic ISSN |
1460-2105 |
Print ISSN |
0027-8874 |
Журнал |
JNCI: Journal of the National Cancer Institute |
Том |
55 |
Первая страница |
1467 |
Последняя страница |
1470 |
Выпуск |
6 |