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Автор Pourreau-Schneider, Natalie
Дата выпуска 1975
dc.description SummaryPropagation of the transplantable acute rat leukemia L5222 in vitro was highly dependent on the reducing agent 2-mercaptoethanol (2-Me). Maximum growth promotion was obtained in the simultaneous presence of 1.25–2.5×10<sup>−4</sup> m 2-Me and 30% fetal bovine serum in enriched tissue culture medium NCTC 135. A short exposure to the reducing agent insured growth of the leukemia cells for 5 days, but extended culture necessitated constant presence of the thiol. After 2–8 days of culture, inoculation of the cell suspension produced widespread leukemia in inbred BD IX rats.
Формат application.pdf
Издатель Oxford University Press
Тема Clinical and Laboratory Investigations on Animals
Название Brief Communication: In Vitro Growth Promotion of Rat Leukemia L5222 Cells in the Presence of 2-MercaptoethanoI2
Тип research-article
DOI 10.1093/jnci/55.6.1467
Electronic ISSN 1460-2105
Print ISSN 0027-8874
Журнал JNCI: Journal of the National Cancer Institute
Том 55
Первая страница 1467
Последняя страница 1470
Выпуск 6

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