| Автор | SUZUKI, Koichi |
| Автор | IMAHORI, Kazutomo |
| Дата выпуска | 1973 |
| dc.description | 1. Glyceraldehyde 3-phosphate dehydrogenase [GPD, EC 1. 2.1.12] from vegetative cells of Bacillus cereus was purified and its molecular and catalytic properties were examined. The purified enzyme was homogeneous as judged by disc gel electro-phoresis with and without SDS and by electrophoresis on cellulose acetate strips at various pH values. The enzyme was a tetramer and the subunit molecular weight was 36, 000 which is exactly the same as that found for GPD from other sources.2. Analysis of SH groups with 5, 5'-dithio-bis(2-nitrobenzoic acid) showed that, in contrast to the enzyme isolated from muscle, there are only 8 SH groups per tetramer (MW 144, 000) of which 4 are highly reactive and essential for activity.3. CD spectra showed that the enzyme has a very high content of β-structure (45%) which seems to be common to other GPDs.4. The enzyme was fairly heat stable and was similar to Bacillus stearothermophilus GPD in many of its properties. |
| Формат | application.pdf |
| Издатель | Oxford University Press |
| Копирайт | © BY THE JOURNAL OF BIOCHEMISTRY |
| Тема | Articles |
| Название | Isolation and Some Properties of Glyceraldehyde 3-Phosphate Dehydrogenase from Vegetative Cells of Bacillus cereus |
| Тип | research-article |
| Electronic ISSN | 1756-2651 |
| Print ISSN | 0021-924X |
| Журнал | Journal of Biochemistry |
| Том | 73 |
| Первая страница | 97 |
| Последняя страница | 106 |
| Аффилиация | Department of Agricultural Chemistry, Faculty of Agriculture, The University of TokyoBunkyo-ku, Tokyo |
| Выпуск | 1 |
