| Автор | pavlov, V.M |
| Автор | Lyubchenko, Yu.L.L. |
| Автор | Borovik, A.S. |
| Автор | Lazurkin, Yu.S. |
| Дата выпуска | 1977 |
| dc.description | A method, has been developed for selective fragmentation of T7 DNA at AT-rich regions. The molecules have been subjected to complete digestion with single-strand-specific SI endonuolease after fixation of DNA AT-rich regions in the denatured state by glyoxal. The treatment resulted in three fragments having molecular weights of 13.6±0.4, 8.2±0.4 and 3.5±0.16 megadaltons as determined by electron microscopy. The position of these fragments along the T7 DNA molecule has been determined by means of analysis of the intermediates during SI-cleavage. |
| Формат | application.pdf |
| Издатель | Oxford University Press |
| Копирайт | © Information Retrieval Limited |
| Тема | Articles |
| Название | Specific fragmentation of T7 phage DNA at low-melting sites |
| Тип | research-article |
| Electronic ISSN | 1362-4962 |
| Print ISSN | 0305-1048 |
| Журнал | Nucleic Acids Research |
| Том | 4 |
| Первая страница | 4053 |
| Последняя страница | 4062 |
| Аффилиация | Department of Biology, I.V.Kurchatov Institute of Atomic EnergyMoscow, USSR |
| Выпуск | 11 |
