| Автор | Ruitenberg, E. J. |
| Автор | van Knapen, F. |
| Дата выпуска | 1977 |
| dc.description | The enzyme-linked immunosorbent assay (ELISA) was used for the serodiagnosis of four parasitic infections. ELISA was performed as a macromethod in polystyrene tubes and/or as a micromethod in polystyrene microplates. Conventionally raised pigs were infected orally with different numbers of Trichinella spiralis larvae, and the pigs' sera were tested for antibody to T. spiralis. Results were compared with those from sera of uninfected conventionally raised pigs. Cynomolgus monkeys were infected orally and immunized with Toxocara canis. The results of macro-ELISA and micro-ELISA were compared with those of immunofluorescence. Micro-ELISA proved to be more sensitive than macro-ELISA, the latter being more sensitive than immunofluorescence. The applicability of ELISA for seroepidemiology was demonstrated by results obtained with sera from patients with proved African trypanosomiasis. With both macro-ELISA and micro-ELISA it was possible to discriminate between the positive and negative sera. ELISA was used for study of the seroepidemiology of toxoplasmosis. Again, positive sera could be distinguished from negative sera. The examples presented show that ELISA can be used as an additional serologic tool for the detection of both helminthic and protozoal infections. |
| Формат | application.pdf |
| Издатель | The University of Chicago Press |
| Копирайт | © 1977 by The University of Chicago |
| Тема | Scientific Articles |
| Название | The Enzyme-Linked Immunosorbent Assay and Its Application to Parasitic Infections |
| Тип | research-article |
| DOI | 10.1093/infdis/136.Supplement_2.S267 |
| Electronic ISSN | 1537-6613 |
| Print ISSN | 0022-1899 |
| Журнал | The Journal of Infectious Diseases |
| Том | 136 |
| Первая страница | S267 |
| Последняя страница | S273 |
| Аффилиация | From the Laboratory of Pathology, National Institute of Public Health, Bilthouen, The Netherlands |
| Выпуск | Supplement_2 |
