| Автор | Honjoh, Ken-ichi |
| Автор | Yoshimoto, Makoto |
| Автор | Joh, Toshio |
| Автор | Kajiwara, Taishin |
| Автор | Miyamoto, Takahisa |
| Автор | Hatano, Shoji |
| Дата выпуска | 1995 |
| dc.description | Hardening-induced soluble proteins of Chlorella vulgaris Beijerink IAM C-27 (formerly Chlorella ellipsoidea Gerneck IAM C-27) were isolated and purified by two-dimensional high-performance liquid chromatography (2D-HPLC) on an anion-exchange column, with subsequent reversed-phase chromatography. Some of the proteins were resolved by SDS-PAGE, characterized by amino-terminal sequencing and identified by searching for homologies in databases. Separation of the soluble proteins during the hardening of Chlorella by a combination of 2D-HPLC and SDS-PAGE revealed that at least 31 proteins were induced or increased in abundance. Of particular interest was the induction after 12 h of a 10-kDa protein with the amino-terminal amino acid sequence AGNKPITEQISDAVGAAGQKVG and the induction after 6 h of a 14-kDa protein with the amino-terminal sequence ALGEESLGDKAKNAFEDAKDAVKDAAGNVKEAV. The amino-terminal sequences of these proteins indicated that they were homologous to late embryogenesis abundant (LEA) proteins. Furthermore, the level of a 22-kDa protein also increased after 12 h. The amino-terminal sequence of this protein, AAPLVGGPAPDFTAAAVFD, indicated that it was homologous to thioredoxin peroxidase. |
| Формат | application.pdf |
| Издатель | Oxford University Press |
| Копирайт | Copyright © 1995. The Japanese Society of Plant Physiologists |
| Тема | Boiling-soluble protein |
| Тема | Chlorella vulgaris C-27 |
| Тема | 2D-HPLC |
| Тема | Freezing tolerance |
| Тема | LEA protein |
| Тема | Regular Papers |
| Название | Isolation and Characterization of Hardening-Induced Proteins in Chlorella vulgaris C-27: Identification of Late Embryogenesis Abundant Proteins |
| Тип | other |
| Electronic ISSN | 1471-9053 |
| Print ISSN | 0032-0781 |
| Журнал | Plant and Cell Physiology |
| Том | 36 |
| Первая страница | 1421 |
| Последняя страница | 1430 |
| Аффилиация | Department of Food Science and Technology, Faculty of Agriculture, Kyushu UniversityFukuoka, 812-81 Japan |
| Выпуск | 8 |
