1-Ethynylpyrene-modified guanine and cytosine as optical labels for DNA hybridization
Wagner, Clemens; Rist, Manuela; Mayer-Enthart, Elke; Wagenknecht, Hans-Achim; Wagner Clemens; Technical University of Munich, Chemistry Department; Rist Manuela; Technical University of Munich, Chemistry Department; Mayer-Enthart Elke; Technical University of Munich, Chemistry Department; Wagenknecht Hans-Achim; Technical University of Munich, Chemistry Department
Журнал:
Organic & Biomolecular Chemistry
Дата:
2005
Аннотация:
1-Ethynylpyrene shows remarkable absorption changes upon DNA hybridization when it is covalently attached to the 8-position of guanine. An absorption band at â ¼420 nm is only present in the duplex, exhibits thermal melting behaviour and provides the basis for a molecular beacon together with 1-ethynylpyrene-modified cytosine.
171.2Кб