| Автор | Nakagawa, Koji |
| Автор | Yamano, Shuji |
| Автор | Nakasaka, Hisayo |
| Автор | Hinokio, Kenji |
| Автор | Yoshizawa, Midori |
| Автор | Aono, Toshihiro |
| Дата выпуска | 2001 |
| dc.description | Parthenogenetic activation with various combinations of the calcium ionophore A23187 and protein synthesis or phosphorylation inhibitors was investigated as a means of producing human parthenogenones with one haploid pronucleus. Unfertilised human aged oocytes exposed to 5 μM A23187 for 5 min were treated with 10 μg/ml puromycin (puromycin group, 46 oocytes) or 2 mM 6-dimethylaminopurine (DMAP group, 42 oocytes) for 5 h. Oocytes treated only with A23187 served as a control (control group, 40 oocytes). After washing the oocytes, they were incubated for up to 37 h. Evidence of activation (pronuclear formation) and cleavage was observed 18 h and 42 h after A23187 treatment, respectively. Activation rates in the puromycin and DMAP groups were significantly higher than in the control group (91% (42/46) and 77% (34/44) vs 20% (8/40), p < 0.05, respectively). In the puromycin group, 81% (34/42) of the activated oocytes showed one pronucleus with the second polar body (2ndPB), whereas none (0/34) of the activated oocytes in the DMAP group extruded the 2ndPB. The cleavage rate in the puromycin group was significantly lower than in the DMAP group (38% vs 68%, p < 0.05). The activated oocytes which had one pronucleus with the 2ndPB in the puromycin group showed a haploid set of chromosomes (10/13). In conclusion, the combination of A23187 and puromycin is effective for producing human parthenogenones with one haploid pronucleus. |
| Издатель | Cambridge University Press |
| Тема | Calcium ionophore A23187 |
| Тема | Haploid pronucleus |
| Тема | Human parthenogenones |
| Тема | Oocyte activation |
| Тема | Puromycin |
| Название | A combination of calcium ionophore and puromycin effectively produces human parthenogenones with one haploid pronucleus |
| DOI | 10.1017/S0967199401001083 |
| Electronic ISSN | 1469-8730 |
| Print ISSN | 0967-1994 |
| Журнал | Zygote |
| Том | 9 |
| Первая страница | 83 |
| Последняя страница | 88 |
| Аффилиация | Nakagawa Koji; School of Medicine, The University of Tokushima; |
| Аффилиация | Yamano Shuji; School of Medicine, The University of Tokushima |
| Аффилиация | Nakasaka Hisayo; School of Medicine, The University of Tokushima |
| Аффилиация | Hinokio Kenji; School of Medicine, The University of Tokushima |
| Аффилиация | Yoshizawa Midori; Faculty of Agriculture, Utsunomiya University |
| Аффилиация | Aono Toshihiro; School of Medicine, The University of Tokushima |
| Выпуск | 1 |
