| Автор | Kishigami, Satoshi |
| Автор | Van Thuan, Nguyen |
| Автор | Wakayama, Sayaka |
| Автор | Hikichi, Takafusa |
| Автор | Wakayama, Teruhiko |
| Дата выпуска | 2004 |
| dc.description | In the current widely used round spermatid injection (ROSI) protocol for the mouse, the spermatid nucleus is separated from most of the cytoplasm before ROSI by drawing a spermatid in and out of a pipette. This results in the highest rate of normal fertilization. However, this separation method is not always consistent and can be time-consuming. An alternative separation method that cuts away the cytoplasm using the tip of an injection pipette was developed. After removing the cytoplasm, ROSI was performed following both post- and pre-activation protocols and development in vitro and in vivo were examined. The new method consistently removed the bulk of the cytoplasm, as shown by quantifying mitochondria. ROSI without the cytoplasm resulted in significantly higher rates of fertilization than ROSI with the cytoplasm into either post- or pre-activated oocytes. Furthermore, the offspring production rates of ROSI without the cytoplasm were also high (50% and 49% for the post- and pre-activation protocols, respectively). This new method for separating the cytoplasm is an alternative way of producing offspring using ROSI. |
| Издатель | Cambridge University Press |
| Тема | Gamete biology |
| Тема | ROSI |
| Тема | Spermatid |
| Название | A novel method for isolating spermatid nuclei from cytoplasm prior to ROSI in the mouse |
| DOI | 10.1017/S0967199404002965 |
| Electronic ISSN | 1469-8730 |
| Print ISSN | 0967-1994 |
| Журнал | Zygote |
| Том | 12 |
| Первая страница | 321 |
| Последняя страница | 327 |
| Аффилиация | Kishigami Satoshi; RIKEN Center for Developmental Biology; RIKEN Center for Developmental Biology |
| Аффилиация | Van Thuan Nguyen; RIKEN Center for Developmental Biology |
| Аффилиация | Wakayama Sayaka; RIKEN Center for Developmental Biology |
| Аффилиация | Hikichi Takafusa; RIKEN Center for Developmental Biology |
| Аффилиация | Wakayama Teruhiko; RIKEN Center for Developmental Biology |
| Выпуск | 4 |
