| Автор | Ponce, Ruben H. |
| Автор | Urch, Umbert A. |
| Автор | Yanagimachi, Ryuzo |
| Дата выпуска | 1994 |
| dc.description | SummaryAfter spermatozoa bind to and penetrate the extracellular matrix of the egg, the zona pellucida, they adhere to and fuse with the plasma membrane of the egg. Since sperm–egg fusion may involve membrane glycoproteins and/or carbohydrate binding proteins, we sought to test this hypothesis by challenging sperm–egg fusion in hamster and in mouse with added carbohydrates. In this study, a number of carbohydrate and glycoconjugates were examined for their ability to inhibit sperm–eggfusion. In the hamster, D(+)-glucosamine, D(+)-galactosamine, albumin-bovine-glucosamide and-galactosamide, fucoidan and dextran sulphate inhibited the fusion of spermatozoa with zona-free eggs. The same effects were seen in the mouse, except for the toxic effects of D(+)-galactosamine. These facts suggest a role of carbohydrate binding proteins or glycoproteins in the fertilisation process at the level of binding to and fusing with the oolemma. |
| Формат | application.pdf |
| Издатель | Cambridge University Press |
| Копирайт | Copyright © Cambridge University Press 1994 |
| Тема | Egg |
| Тема | Fertilisation |
| Тема | Membrane fusion |
| Тема | Spermatozoa |
| Название | Inhibition of sperm-egg fusion in the hamster and mouse by carbohydrates |
| Тип | research-article |
| DOI | 10.1017/S0967199400002057 |
| Electronic ISSN | 1469-8730 |
| Print ISSN | 0967-1994 |
| Журнал | Zygote |
| Том | 2 |
| Первая страница | 253 |
| Последняя страница | 262 |
| Аффилиация | Ponce Ruben H.; University of california |
| Аффилиация | Urch Umbert A.; University of california |
| Аффилиация | Yanagimachi Ryuzo; University of california |
| Выпуск | 3 |
