| Автор | Koo, Ok Jae |
| Автор | Hossein, Mohammad Shamim |
| Автор | Hong, So Gun |
| Автор | Martinez-Conejero, Jose A. |
| Автор | Lee, Byeong Chun |
| dc.description | SummaryCycle synchronization of donor cells in the G<sub>0</sub>/G<sub>1</sub> stage is a crucial step for successful somatic cell nuclear transfer. In the present report, we evaluated the effects of contact inhibition, serum starvation and the reagents – dimethyl sulphoxide (DMSO), roscovitine and cycloheximide (CHX) – on synchronization of canine fibroblasts at the G<sub>0</sub>/G<sub>1</sub> stage. Ear fibroblast cells were collected from a beagle dog, placed into culture and used for analysis at passages three to eight. The population doubling time was 36.5 h. The proportion of G<sub>0</sub>/G<sub>1</sub> cells was significantly increased by contact inhibition (77.1%) as compared with cycling cells (70.1%); however, extending the duration of culture did not induce further synchronization. After 24 h of serum starvation, cells were effectively synchronized at G<sub>0</sub>/G<sub>1</sub> (77.1%). Although synchronization was further increased gradually after 24 h and even showed significant difference after 72 h (82.8%) of starvation, the proportion of dead cells also significantly increased after 24 h. The percentage of cells at the G<sub>0</sub>/G<sub>1</sub> phase was increased (as compared with controls) after 72 h treatment with DMSO (76.1%) and after 48 h treatment with CHX (73.0%) or roscovitine (72.5%). However, the rate of cell death was increased after 24 and 72 h of treatment with DMSO and CHX, respectively. Thus, we recommend the use of roscovitine for cell cycle synchronization of canine ear fibroblasts as a preparatory step for SCNT. |
| Формат | application.pdf |
| Издатель | Cambridge University Press |
| Копирайт | Copyright © Cambridge University Press 2008 |
| Тема | Canine |
| Тема | Cell cycle synchronization |
| Тема | Ear fibroblast |
| Тема | Roscovitine |
| Тема | Somatic cell nuclear transfer |
| Название | Cell cycle synchronization of canine ear fibroblasts for somatic cell nuclear transfer |
| Тип | research-article |
| DOI | 10.1017/S096719940800498X |
| Electronic ISSN | 1469-8730 |
| Print ISSN | 0967-1994 |
| Журнал | Zygote |
| Том | 17 |
| Первая страница | 37 |
| Последняя страница | 43 |
| Аффилиация | Koo Ok Jae; Seoul National University |
| Аффилиация | Hossein Mohammad Shamim; Seoul National University |
| Аффилиация | Hong So Gun; Seoul National University |
| Аффилиация | Martinez-Conejero Jose A.; Valencia University |
| Аффилиация | Lee Byeong Chun; Seoul National University |
| Выпуск | 1 |
