| Автор | Molnarova, Zuzana |
| Автор | Machatkova, Marie |
| Автор | Machal, Ladislav |
| Автор | Horakova, Jindra |
| Автор | Hanzalova, Katerina |
| Дата выпуска | 2006 |
| dc.description | The aim of the work was to study a potential relationship between acrosome response characteristics of bovine spermatozoa and their ability to fertilize oocytes and produce in vitro embryos. Sperm of artificial insemination bulls with a high rate (22.0 ± 4.1%, group A, n = 7) or a low rate (10.3 ± 4.1%, group B, n = 8) of embryos were used. For acrosome assessment, motile spermatozoa from a Percoll gradient were incubated with or without heparin and examined by the fix-vital sperm assay (FVSA). The differences between the heparin-treated (H<sup>+</sup>) and the non-treated (H<sup>−</sup>) spermatozoa were significant (p < 0.01) in all bulls at all tested intervals. According to the kinetics of the heparin response, the bulls fell into three categories: fast (FR, n = 7), moderate (MR, n = 5) or slow (SR, n = 3) acrosome responses (p < 0.01). Five MR bulls were found in group A in comparison with two MR bulls in group B (57.1 vs 12.5%; p < 0.05). Intensity of the acrosome response (response index) was significantly higher in bull group A compared with bull group B (7.0 vs 4.6, p < 0.01). A positive correlation was recorded between response index and embryo rate (r = 0.668, p < 0.01). In conclusion (a) the kinetics of spermatozoa response to heparin may be important for in vitro fertilization, bulls with a moderate response appearing to be most suitable for embryo production; (b) greater spermatozoa response to heparin was related to more effective embryo production. |
| Издатель | Cambridge University Press |
| Тема | Acrosome |
| Тема | Bulls |
| Тема | Embryos |
| Тема | Heparin |
| Тема | Spermatozoa |
| Название | A potential relationship between the acrosome response characteristics of bovine spermatozoa and their in vitro fertilizing ability |
| DOI | 10.1017/S096719940600356X |
| Electronic ISSN | 1469-8730 |
| Print ISSN | 0967-1994 |
| Журнал | Zygote |
| Том | 14 |
| Первая страница | 63 |
| Последняя страница | 69 |
| Аффилиация | Molnarova Zuzana; Mendel University of Agriculture and Forestry |
| Аффилиация | Machatkova Marie; Veterinary Research Institute; Veterinary Research Institute |
| Аффилиация | Machal Ladislav; Mendel University of Agriculture and Forestry |
| Аффилиация | Horakova Jindra; Veterinary Research Institute |
| Аффилиация | Hanzalova Katerina; Veterinary Research Institute |
| Выпуск | 1 |
