| Автор | Yu, Jian-Ning |
| Автор | Xue, Chun-Yang |
| Автор | Wang, Xu-Guang |
| Автор | Lin, Fei |
| Автор | Liu, Chun-Yi |
| Автор | Lu, Fu-Zeng |
| Автор | Liu, Hong-Lin |
| dc.description | Summary5-AZA-2′-deoxycytidine (5-AZA-CdR) is a demethylating, teratogenic agent and a mutagen, which causes defects in the developing mouse and rat after implantation. Our previous data indicated that 5-AZA-CdR (0.2 and 1.0 μM) inhibited the development of mouse preimplantation embryos. Pronuclear embryos exposed to 5-AZA-CdR at the pronuclear stage were unable to form 8-cell embryos, while 2-cell-stage embryos exposed to 5-AZA-CdR only developed into uncompacted 8-cell-stage embryos. And there was no formation of blastocysts when 4-cell embryos cultured in 5-AZA-CdR. In our present study, we detected Dnmt1o protein and some developmental gene expression in order to find the reasons for the developmental arrest. Dnmt1o could not traffic to 8-cell nuclei as control when embryos were exposed to 5-AZA-CdR. Dnmt1o was in cytoplasm at 2-cell and 4-cell stages before and after treated with 5-AZA-CdR. Gene expression changes were also detected in this research. Our data indicated that connexin 31 (Cx31), connexin 43 (Cx43), connexin 45 (Cx45), E-cadherin (Cdh1) and β-catenin (Ctnnb1) were all downregulated by 5-AZA-CdR. Cx31, Cx43 and Cx45 are members of connexins family, which have a central role in gap junctions. Cdh1 and Ctnnb1 are necessary for the foundation of tight junctions. Therefore, developmental arrest induced by 5-AZA-CdR may be caused by the failure of Dnmt1o cytoplasmic–nuclear traffic and the down-regulation of developmental gene expression. Normal compaction and blastocoel cavitation need Dnmt1o traffic to 8-cell nuclei and the right gene expression, especially the correlative genes in gap junctions and tight junctions. |
| Формат | application.pdf |
| Издатель | Cambridge University Press |
| Копирайт | Copyright © Cambridge University Press 2009 |
| Тема | 5-AZA-CdR |
| Тема | DNA methylation |
| Тема | Dnmt1o |
| Тема | Gene expression |
| Тема | Preimplantation mouse embryos |
| Название | 5-AZA-2′-deoxycytidine (5-AZA-CdR) leads to down-regulation of Dnmt1o and gene expression in preimplantation mouse embryos |
| Тип | research-article |
| DOI | 10.1017/S0967199408005169 |
| Electronic ISSN | 1469-8730 |
| Print ISSN | 0967-1994 |
| Журнал | Zygote |
| Том | 17 |
| Первая страница | 137 |
| Последняя страница | 145 |
| Аффилиация | Yu Jian-Ning; Nanjing Agricultural University; Institute of Animal Science |
| Аффилиация | Xue Chun-Yang; Nanjing Agricultural University |
| Аффилиация | Wang Xu-Guang; Nanjing Agricultural University |
| Аффилиация | Lin Fei; Nanjing Agricultural University |
| Аффилиация | Liu Chun-Yi; Nanjing Agricultural University |
| Аффилиация | Lu Fu-Zeng; Nanjing Agricultural University |
| Аффилиация | Liu Hong-Lin; Nanjing Agriculture University |
| Выпуск | 2 |
