A method for characterizing adsorption of flowing solutes to microfluidic device surfaces
Hawkins, Kenneth R.; Steedman, Mark R.; Baldwin, Richard R.; Fu, Elain; Ghosal, Sandip; Yager, Paul; Hawkins Kenneth R.; Department of Bioengineering, University of Washington; Steedman Mark R.; UCSF/UC Berkeley Joint Graduate Group in Bioengineering, University of California at San Francisco and Berkeley; Baldwin Richard R.; Department of Bioengineering, University of Washington; Fu Elain; Department of Bioengineering, University of Washington; Ghosal Sandip; Department of Mechanical Engineering, Northwestern University; Yager Paul; Department of Bioengineering, University of Washington
Журнал:
Lab on a Chip
Дата:
2007
Аннотация:
We present a method for characterizing the adsorption of solutes in microfluidic devices that is sensitive to both long-lived and transient adsorption and can be applied to a variety of realistic device materials, designs, fabrication methods, and operational parameters. We have characterized the adsorption of two highly adsorbing molecules (FITC-labeled bovine serum albumin (BSA) and rhodamine B) and compared these results to two low adsorbing species of similar molecular weights (FITC-labeled dextran and fluorescein). We have also validated our method by demonstrating that two well-known non-fouling strategies [deposition of the polyethylene oxide (PEO)-like surface coating created by radio-frequency glow discharge plasma deposition (RF-GDPD) of tetraethylene glycol dimethyl ether (tetraglyme, CH3O(CH2CH2O)4CH3), and blocking with unlabeled BSA] eliminate the characteristic BSA adsorption behavior observed otherwise.
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