Physical properties of gravitational instantons which are derivable from minimal surfaces in three-dimensional Euclidean space are examined using the Newman-Penrose formalism for Euclidean signature. The gravitational instanton that corresponds to the helicoid minimal surface is investigated in detail. This is a metric of Bianchi type , or E(2), which admits a hidden symmetry due to the existence of a quadratic Killing tensor. It leads to a complete separation of variables in the Hamilton-Jacobi equation for geodesics, as well as in Laplace's equation for a massless scalar field. The scalar Green function can be obtained in closed form, which enables us to calculate the vacuum fluctuations of a massless scalar field in the background of this instanton.