A New Method for Determining Dipole Moment Orientation of Single Molecules
Wang Chen; Liu Li; Wang Gui-Ying; Xu Zhi-Zhan; Wang Chen; Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800; Liu Li; Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800; Wang Gui-Ying; Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800; Xu Zhi-Zhan; Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800
Журнал:
Chinese Physics Letters
Дата:
2004-05-01
Аннотация:
A new imaging method is proposed to determine the three-dimensional dipole moment orientation of single fluorophore. Far-field microscopy can provide orientational information projected in the sample plane, while total internal reflection fluorescence microscopy (TIRFM) can offer the knowledge perpendicular to the surface because longitudinal electric-field components can be generated in total internal reflection geometry. By comparing fluorescence intensities measured with far-field epi-fluorescence microscopy and TIRFM, the exact information of single-fluorescent-molecule orientation is extracted. Detailed analysis of the method is given with a numerical example.
338.7Кб