| Автор | Wang Chen |
| Автор | Liu Li |
| Автор | Wang Gui-Ying |
| Автор | Xu Zhi-Zhan |
| Дата выпуска | 2004-05-01 |
| dc.description | A new imaging method is proposed to determine the three-dimensional dipole moment orientation of single fluorophore. Far-field microscopy can provide orientational information projected in the sample plane, while total internal reflection fluorescence microscopy (TIRFM) can offer the knowledge perpendicular to the surface because longitudinal electric-field components can be generated in total internal reflection geometry. By comparing fluorescence intensities measured with far-field epi-fluorescence microscopy and TIRFM, the exact information of single-fluorescent-molecule orientation is extracted. Detailed analysis of the method is given with a numerical example. |
| Формат | application.pdf |
| Издатель | Institute of Physics Publishing |
| Название | A New Method for Determining Dipole Moment Orientation of Single Molecules |
| Тип | paper |
| DOI | 10.1088/0256-307X/21/5/022 |
| Electronic ISSN | 1741-3540 |
| Print ISSN | 0256-307X |
| Журнал | Chinese Physics Letters |
| Том | 21 |
| Первая страница | 843 |
| Последняя страница | 845 |
| Аффилиация | Wang Chen; Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800 |
| Аффилиация | Liu Li; Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800 |
| Аффилиация | Wang Gui-Ying; Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800 |
| Аффилиация | Xu Zhi-Zhan; Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800 |
| Выпуск | 5 |
