Polyaniline films were prepared by electropolymerization under a magnetic field of 5T parallel (+5T) or antiparallel (−5T) to the faradaic currents. They were used as a modified electrode and their chiral properties were examined for L-3-(3,4-dihydroxyphenyl)alanine (L-DOPA), L-ascorbic acid (L-AA) and D-isoascorbic acid (D-AA) by cyclic voltammetry. The redox currents of L-DOPA on the +5T-film electrode were smaller than those on the −5T-film electrode, and the results for racemic mixture of L- and D-DOPA were the same on the two electrodes. The oxidation current of L-AA on the +5T-film electrode was larger than that on the −5T-film electrode, and the result for D-AA was opposite. These facts demonstrate that the two electrodes have the opposite chirality each other and possess the ability of chiral recognition.