A localized surface plasmon resonance based immunosensor for the detection of casein in milk
Minh Hiep, Ha; Endo, Tatsuro; Kerman, Kagan; Chikae, Miyuki; Kim, Do-Kyun; Yamamura, Shohei; Takamura, Yuzuru; Tamiya, Eiichi; Minh Hiep, Ha; School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1, Asahidai, Nomi City, Ishikawa 923-1292, Japan; Endo, Tatsuro; Department of Mechano-Micro Engineering, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8502, Japan; Kerman, Kagan; School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1, Asahidai, Nomi City, Ishikawa 923-1292, Japan; Chikae, Miyuki; School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1, Asahidai, Nomi City, Ishikawa 923-1292, Japan; Kim, Do-Kyun; School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1, Asahidai, Nomi City, Ishikawa 923-1292, Japan; Yamamura, Shohei; School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1, Asahidai, Nomi City, Ishikawa 923-1292, Japan; Takamura, Yuzuru; School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1, Asahidai, Nomi City, Ishikawa 923-1292, Japan; Tamiya, Eiichi; School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1, Asahidai, Nomi City, Ishikawa 923-1292, Japan;
Журнал:
Science and Technology of Advanced Materials
Дата:
2007-05-31
Аннотация:
In this research, a localized surface plasmon resonance (LSPR) immunosensor based on gold-capped nanoparticle substrate for detecting casein, one of the most potent allergens in milk, was developed. The fabrication of the gold-capped nanoparticle substrate involved a surface-modified silica nanoparticle layer (core) on the slide glass substrate between bottom and top gold layers (shell). The absorbance peak of the gold-capped nanoparticle substrate was observed at ∼520 nm. In addition, the atomic force microscopy (AFM) images demonstrated that the nanoparticles formed a monolayer on the slide glass. After immobilizing anti-casein antibody on the surface, our device, casein immunosensor, could be applied easily for the detection of casein in the raw milk sample without a difficult pretreatment. Under the optimum conditions, the detection limit of the casein immunosensor was determined as 10 ng/mL. Our device brings several advantages to the existing LSPR-based biosensors with its easy fabrication, simple handling, low-cost, and high sensitivity.
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