| Автор | Minh Hiep, Ha |
| Автор | Endo, Tatsuro |
| Автор | Kerman, Kagan |
| Автор | Chikae, Miyuki |
| Автор | Kim, Do-Kyun |
| Автор | Yamamura, Shohei |
| Автор | Takamura, Yuzuru |
| Автор | Tamiya, Eiichi |
| Дата выпуска | 2007-05-31 |
| dc.description | In this research, a localized surface plasmon resonance (LSPR) immunosensor based on gold-capped nanoparticle substrate for detecting casein, one of the most potent allergens in milk, was developed. The fabrication of the gold-capped nanoparticle substrate involved a surface-modified silica nanoparticle layer (core) on the slide glass substrate between bottom and top gold layers (shell). The absorbance peak of the gold-capped nanoparticle substrate was observed at ∼520 nm. In addition, the atomic force microscopy (AFM) images demonstrated that the nanoparticles formed a monolayer on the slide glass. After immobilizing anti-casein antibody on the surface, our device, casein immunosensor, could be applied easily for the detection of casein in the raw milk sample without a difficult pretreatment. Under the optimum conditions, the detection limit of the casein immunosensor was determined as 10 ng/mL. Our device brings several advantages to the existing LSPR-based biosensors with its easy fabrication, simple handling, low-cost, and high sensitivity. |
| Формат | application.pdf |
| Издатель | Institute of Physics Publishing |
| Копирайт | © 2007 NIMS and Elsevier Ltd |
| Название | A localized surface plasmon resonance based immunosensor for the detection of casein in milk |
| Тип | paper |
| DOI | 10.1016/j.stam.2006.12.010 |
| Electronic ISSN | 1878-5514 |
| Print ISSN | 1468-6996 |
| Журнал | Science and Technology of Advanced Materials |
| Том | 8 |
| Первая страница | 331 |
| Последняя страница | 338 |
| Аффилиация | Minh Hiep, Ha; School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1, Asahidai, Nomi City, Ishikawa 923-1292, Japan |
| Аффилиация | Endo, Tatsuro; Department of Mechano-Micro Engineering, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8502, Japan |
| Аффилиация | Kerman, Kagan; School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1, Asahidai, Nomi City, Ishikawa 923-1292, Japan |
| Аффилиация | Chikae, Miyuki; School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1, Asahidai, Nomi City, Ishikawa 923-1292, Japan |
| Аффилиация | Kim, Do-Kyun; School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1, Asahidai, Nomi City, Ishikawa 923-1292, Japan |
| Аффилиация | Yamamura, Shohei; School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1, Asahidai, Nomi City, Ishikawa 923-1292, Japan |
| Аффилиация | Takamura, Yuzuru; School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1, Asahidai, Nomi City, Ishikawa 923-1292, Japan |
| Аффилиация | Tamiya, Eiichi; School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1, Asahidai, Nomi City, Ishikawa 923-1292, Japan; |
| Выпуск | 4 |
