| Автор | MAUDET, CÉLIA |
| Автор | TABERLET, PIERRE |
| Дата выпуска | 2001 |
| dc.description | A new polymerase chain reaction (PCR)-based method was developed to detect cowsʼ milk in goat cheese. This method is based on mitochondrial DNA (mtDNA) control region sequence variations. DNA extractions from 150 mg of cheese were carried out using a spin column-based method. Subsequent PCR amplifications of DNA were performed with cow specific primers, demonstrating the ability to detect cowsʼ milk in a variety of cheeses. This simple approach provides high quality DNA, and is shown to be very sensitive, with a detection limit of less than 0·1% of cowsʼ milk. Analysis of an agarose gel digital image allows a rough estimation of the percentage of cowsʼ milk used in adulteration. |
| Издатель | Cambridge University Press |
| Тема | Goatsʼ milk |
| Тема | cheese |
| Тема | cowsʼ milk |
| Тема | detection |
| Тема | PCR |
| Тема | mitochondrial DNA |
| Название | Detection of cowsʼ milk in goatsʼ cheeses inferred from mitochondrial DNA polymorphism |
| DOI | doi:10.1017/S0022029901004794 |
| Electronic ISSN | 1469-7629 |
| Print ISSN | 0022-0299 |
| Журнал | Journal of Dairy Research |
| Том | 68 |
| Первая страница | 229 |
| Последняя страница | 235 |
| Аффилиация | MAUDET CÉLIA; Laboratoire de Biologie des Populations dʼAltitude; |
| Аффилиация | TABERLET PIERRE; Laboratoire de Biologie des Populations dʼAltitude |
| Выпуск | 2 |
