A cell population structuring model to estimate recombinant strain growth in a closed system for subsequent search of the mode to increase protein accumulation during protealysin producer cultivation
Klykov, S P; Kurakov, V V; Vilkov, V B; Demidyuk, I V; Gromova, T Yu; Skladnev, D A; Klykov, S P; PHARM-REGION, Ltd, c.1, b.10, Baryshikha street, Moscow, 125222, Russia;; Kurakov, V V; PHARM-REGION, Ltd, c.1, b.10, Baryshikha street, Moscow, 125222, Russia; Vilkov, V B; PHARM-REGION, Ltd, c.1, b.10, Baryshikha street, Moscow, 125222, Russia; Demidyuk, I V; Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov Sq. 2, Moscow 123182, Russia; Gromova, T Yu; Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov Sq. 2, Moscow 123182, Russia; Skladnev, D A; PHARM-REGION, Ltd, c.1, b.10, Baryshikha street, Moscow, 125222, Russia
Журнал:
Biofabrication
Дата:
2011-12-01
Аннотация:
In this paper we have proposed a new structured population growth model, further developing a model previously proposed by the authors. Based on this model, optimal growth characteristics of the recombinant strain Escherichia coli BL-21 (DE3) [pProPlnHis<sub>6</sub>] were determined, which allowed us to increase the output of metalloproteinase by 300%. We have experimentally demonstrated the applicability of the new model to cell cultures with implanted plasmids and the potential practical use for an output increase of a wide variety of biosynthesis processes.
1.019Мб