| Автор | Klykov, S P |
| Автор | Kurakov, V V |
| Автор | Vilkov, V B |
| Автор | Demidyuk, I V |
| Автор | Gromova, T Yu |
| Автор | Skladnev, D A |
| Дата выпуска | 2011-12-01 |
| dc.description | In this paper we have proposed a new structured population growth model, further developing a model previously proposed by the authors. Based on this model, optimal growth characteristics of the recombinant strain Escherichia coli BL-21 (DE3) [pProPlnHis<sub>6</sub>] were determined, which allowed us to increase the output of metalloproteinase by 300%. We have experimentally demonstrated the applicability of the new model to cell cultures with implanted plasmids and the potential practical use for an output increase of a wide variety of biosynthesis processes. |
| Формат | application.pdf |
| Издатель | Institute of Physics Publishing |
| Копирайт | 2011 IOP Publishing Ltd |
| Название | A cell population structuring model to estimate recombinant strain growth in a closed system for subsequent search of the mode to increase protein accumulation during protealysin producer cultivation |
| Тип | paper |
| DOI | 10.1088/1758-5082/3/4/045006 |
| Electronic ISSN | 1758-5090 |
| Print ISSN | 1758-5082 |
| Журнал | Biofabrication |
| Том | 3 |
| Первая страница | 45006 |
| Последняя страница | 45017 |
| Аффилиация | Klykov, S P; PHARM-REGION, Ltd, c.1, b.10, Baryshikha street, Moscow, 125222, Russia; |
| Аффилиация | Kurakov, V V; PHARM-REGION, Ltd, c.1, b.10, Baryshikha street, Moscow, 125222, Russia |
| Аффилиация | Vilkov, V B; PHARM-REGION, Ltd, c.1, b.10, Baryshikha street, Moscow, 125222, Russia |
| Аффилиация | Demidyuk, I V; Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov Sq. 2, Moscow 123182, Russia |
| Аффилиация | Gromova, T Yu; Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov Sq. 2, Moscow 123182, Russia |
| Аффилиация | Skladnev, D A; PHARM-REGION, Ltd, c.1, b.10, Baryshikha street, Moscow, 125222, Russia |
| Выпуск | 4 |
