| Автор | Mills, E. N. Clare |
| Автор | Spinks, Caroline A. |
| Автор | Morgan, Michael R. A. |
| Дата выпуска | 1989 |
| dc.description | A two site enzyme‐linked immunosorbent assay for wheat gliadins has been developed. Polyclonal antibodies from chicken eggs were used as the capture agent adsorbed to the surface of the microtitration plate. Murine monoclonal antibodies bound to captured gliadin were detected by addition of enzyme‐labelled, species‐specific antibodies. The assay was developed for the purposes of screening monoclonal antibodies specific for conformational epitopes on gliadins. The properties of a panel of 6 anti‐gliadin antibodies in the assay were assessed and compared to properties exhibited under conditions where conformational epitopes were less likely to be present. Three of the monoclonal antibodies appeared to be specific for conformational epitopes. |
| Формат | application.pdf |
| Издатель | Taylor & Francis Group |
| Копирайт | Copyright Taylor and Francis Group, LLC |
| Название | A two‐site enzyme‐linked immunosorbent assay for wheat gliadins |
| Тип | research-article |
| DOI | 10.1080/09540108909354671 |
| Electronic ISSN | 1465-3443 |
| Print ISSN | 0954-0105 |
| Журнал | Food and Agricultural Immunology |
| Том | 1 |
| Первая страница | 19 |
| Последняя страница | 27 |
| Аффилиация | Mills, E. N. Clare; Norwich Laboratory, AFRC Institute of Food Research |
| Аффилиация | Spinks, Caroline A.; Norwich Laboratory, AFRC Institute of Food Research |
| Аффилиация | Morgan, Michael R. A.; Norwich Laboratory, AFRC Institute of Food Research |
| Выпуск | 1 |
| Библиографическая ссылка | Ayob, M. K., Rittenburg, J., Allen, J. C. and Smith, C. J. 1988. Development of a rapid enzymelinked immunosorbent assay (ELISA) for gliadin determination in food. Food Hydrocolloids, 2: 39–49. |
| Библиографическая ссылка | Berzofsky, J. A. 1985. Intrinsic and extrinsic factors in protein antigenic structure. Science, 229: 932–940. |
| Библиографическая ссылка | Freedman, A. R., Galfre, G., Gal, E., Ellis, H. J. and Ciclitira, P. J. 1987. Monoclonal antibody ELISA to quantitate wheat gliadin contamination of gluten‐free foods. J. Immunol. Methods, 98: 123–127. |
| Библиографическая ссылка | Friguet, B., Djavadi‐Ohaniance, L. and Goldberg, M. E. 1984. Some monoclonal antibodies raised with native protein bind preferentially to the denatured antigen. Molecular Immunol, 21: 673–677. |
| Библиографическая ссылка | Galfre, G., Milstein, C. and Van Vunakis, H. 1981. “Preparation of monoclonal antibodies: strategies and procedures”. In Lethods in Enzymology Volume 73. Immunochemical Techniques (part C), Edited by: Moplangone, J. J. 3–46. New York: Academic Press. |
| Библиографическая ссылка | Jensenius, J. C., Andersen, I., Hau, J., Crone, M. and Koch, C. 1981. Eggs: conveniently packaged antibodies. Methods for purification of yolk lgG. J. Immunol. Methods, 46: 63–68. |
| Библиографическая ссылка | Kilshaw, P. J., McEwan, F. J., Baker, K. C. and Cant, A. J. 1986. Studies on the specificity of antibodies to ovalbumin in normal human serum: technical considerations in the use of ELISA methods. Clin. Exp. Immunol., 66: 481–489. |
| Библиографическая ссылка | Miflin, B. J., Field, J. M. and Shewry, P. R. 1983. “Cereal storage proteins and their effect on technological properties”. In Seed Proteins, Edited by: Dopdaussant, J., Mosse, J. and Vaughan, J. 255–319. New York: Academic Press. |
| Библиографическая ссылка | Moudallal, Z. A., Altsuchuh, D., Briand, J. P. and Van Regenmortel, M. H. V. 1984. Comparative sensitivity of different ELISA procedures for detecting monoclonal antibodies. J. Immunol. Methods, 68: 35–43. |
| Библиографическая ссылка | Shewry, P. R., Miflin, B. J., Lew, E. J‐L. and Kasarda, D. D. 1983. The preparation and characterization of an aggregated gliadin fraction from wheat. J. Exp. Botany, 34: 1403–1410. |
| Библиографическая ссылка | Tatham, A. S. and Shewry, P. R. 1985. The conformation of wheat gluten proteins, The secondary structures and thermal stabilities of α,β,γ‐ and ω‐gliadins. J. Cereal Science, 3: 103–113. |
| Библиографическая ссылка | Tatham, A. S., Miflin, B. J. and Shewry, P. R. 1985. The beta‐turn conformation in wheat gluten proteins: relationship to gluten elasticity. Cereal Chem., 62: 405–412. |
| Библиографическая ссылка | Troncone, R., Vitale, M., Donatiello, A., Farris, E., Rossie, G. and Auricchio, S. 1986. A sandwich immunoassay for wheat gliadin. J. Immunol. Methods, 92: 21–23. |
| Библиографическая ссылка | Vaidya, H. C., Dietzler, D. N. and Ladenson, J. H. 1985. Inadequacy of traditional ELISA for screening hybridoma supernatants for murine monoclonal antibodies. Hybridoma, 4: 271–276. |
| Библиографическая ссылка | Windemann, H., Fritschy, F. and Baumgartner, E. 1982. Enzyme‐linked immunosorbent assay for wheat α‐gliadin and whole gliadin. Biochim. Biophys. Acta, 709: 110–121. |
