A Sensitive Streptavidin‐Biotin Enzyme‐linked Immunosorbent Assay for Rapid Screening of Residues of Chloramphenicol in Milk
Van de water, C.; Haagsma, N.; Van de water, C.; Department of the Science of Food of Animal Origin, Section Chemistry, Faculty of Veterinary Medicine, The University of Utrecht; Haagsma, N.; Department of the Science of Food of Animal Origin, Section Chemistry, Faculty of Veterinary Medicine, The University of Utrecht
Журнал:
Food and Agricultural Immunology
Дата:
1990
Аннотация:
A sensitive streptavidin‐biotin enzyme‐linked immunosorbent assay (ELISA) for the detection of chloramphenicol (CAP) in milk is described. This test is based on a procedure developed earlier for the direct detection of CAP in crude aqueous meat extracts. Milk samples were defatted by centrifugation, filtered and directly submitted to the ELISA procedure. The results were compared with the values obtained by analysis of a part of the sample from which CAP was removed by an immobilized monoclonal antibody preparation. In spiked samples the presence of CAP at concentrations of 1·0 μg/kg and higher can be easily demonstrated. The possibility of the interpretation of the ELISA results with a statistical method, i.e. the so‐called standard deviation ratio (SDR) procedure, was also investigated.
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