An enzyme‐linked immunosorbent assay for deoxynivalenol in wheat, utilizing novel hapten derivatization procedures
Mills, E. N. Clare; Alcock, Sandra M.; Lee, Heather A.; Morgan, Michael R. A.; Mills, E. N. Clare; AFRC Institute of Food Research; Alcock, Sandra M.; AFRC Institute of Food Research; Department of Applied Biochemistry and Food Science, University of Nottingham; Lee, Heather A.; AFRC Institute of Food Research; Morgan, Michael R. A.; AFRC Institute of Food Research
Журнал:
Food and Agricultural Immunology
Дата:
1990
Аннотация:
Polyclonal anti‐deoxynivalenol (vomitoxin) antisera was produced in rabbits. The immunogen was synthesized by using acetyl esterase to deacylate 3‐acetyldeoxynivalenol hemiglutarate and coupling the product to bovine serum albumin using the mixed anhydride reaction. The antiserum was very specific for deoxynivalenol in a microtitration plate ELISA that had a limit of detection of 90 pg per well. A simple, rapid extraction procedure was employed to enable the ELISA to be used for analysis of the deoxynivalenol in wheat.
542.3Кб