| Автор | Alcock, Sandra C. |
| Автор | Finglas, Paul M. |
| Автор | Morgan, Michael R. A. |
| Дата выпуска | 1990 |
| dc.description | A pyridoxal‐protein conjugate was used as an immunogen to raise antisera in rabbits. All sera recognized pyridoxamine strongly and specifically, except one which also recognized pyridoxol. An enzyme‐linked immunosorbent assay (ELISA) for pyridoxamine was set up using the microtitation plate format. Comparison of results obtained with the ELISA on 11 different food samples (ELISA limit of detection 7 pg per well) with those obtained with high performance liquid chromatography (HPLC) showed good agreement except for beef and chicken samples. Comparison of HPLC results with those obtained by microbiological procedures also showed good agreement except for potato and cabbage samples. Food table data for samples at higher levels of B6 did not show good agreement with data derived by HPLC or microbiological procedures. |
| Формат | application.pdf |
| Издатель | Taylor & Francis Group |
| Копирайт | Copyright Taylor and Francis Group, LLC |
| Тема | pyridoxamine |
| Тема | ELISA |
| Тема | vitamin B6 |
| Тема | food |
| Название | An enzyme‐linked immunosorbent assay for pyridoxamine and its comparison with alternative analytical procedures |
| Тип | research-article |
| DOI | 10.1080/09540109009354721 |
| Electronic ISSN | 1465-3443 |
| Print ISSN | 0954-0105 |
| Журнал | Food and Agricultural Immunology |
| Том | 2 |
| Первая страница | 197 |
| Последняя страница | 204 |
| Аффилиация | Alcock, Sandra C.; AFRC Institute of Food Research |
| Аффилиация | Finglas, Paul M.; AFRC Institute of Food Research |
| Аффилиация | Morgan, Michael R. A.; AFRC Institute of Food Research |
| Выпуск | 4 |
| Библиографическая ссылка | Brubacher, G., Muller‐Mulot, W. and Southgate, D. A. T., eds. 1985. Methods for the determination of Vitamins in Food Recommended by Cost 91., London: Elsevier. |
| Библиографическая ссылка | Cordoba, F., Gonzalez, C. and Rivera, P. 1966. Antibodies against pyridoxal‐5'‐phosphate and pyridoxamine‐5'‐phosphate. Biochimica et Biophysica Acta, 127: 151–158. |
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| Библиографическая ссылка | Reynolds, R. D., Leklem, J. E., Leklem, J. E. and Reynolds, R. D., eds. 1981. “Recommended methods for vitamin B6 analysis”. In Methods in Vitamin B6 Nutrition Analysis and Status Assessment, New York: Plenum Press. |
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| Библиографическая ссылка | Vanderslice, J. T., Brownlee, S. R. and Cortissoz, M. E. 1984. Liquid chromatographic determination of vitamin B6 in foods. Journal of the Association of Official Analytical Chemists, 67: 999–1007. |
| Библиографическая ссылка | Vanderslice, J. T., Brownlee, S. G., Cortissoz, M. E. and Maire, C. E. 1985. “Vitamin B6 analysis: sample preparation, extraction procedures, and chromatographic separations”. In Modern Chromatographic Analysis of the Vitamins, Edited by: De Leenheer, A. P., Lambert, W. E. and De Reyter, M. G. M. 435–476. New York: Marcel Dekker. |
| Библиографическая ссылка | Viceps‐Madore, D., Cidlowski, J. A., Kittler, J. M. and Thanassi, J. W. 1983. Preparation, characterization, and use of monoclonal antibodies to vitamin B6. The Journal of Biological Chemistry, 258: 2689–2696. |
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