| Автор | Burianová, T. |
| Автор | Sajdok, J. |
| Автор | Káš, J. |
| Дата выпуска | 1991 |
| dc.description | Cellulase can be added to animal feed mixture for the improvement of nutrient conversion. An enzyme‐linked immunosorbent assay has been developed to check the concentration of added enzyme. The IgG fraction from rabbit immune serum against cellulase from Trichoderma viride was labelled with horseradish peroxidase by the glutaraldehyde method. The conjugate (IgG‐Px) was characterized by gel chromato‐graphy on a Superose 12 column. The assay was optimized with respect to the amount of IgG‐Px applied and the reaction conditions. The method developed was utilized for the assay of feed granules supplemented by cellulase preparations. It is possible under the conditions described to determine added cellulase up to a concentration of 24.0 μg/g of the feed mixture. |
| Формат | application.pdf |
| Издатель | Taylor & Francis Group |
| Копирайт | Copyright Taylor and Francis Group, LLC |
| Тема | enzyme‐linked immunosorbent assay |
| Тема | cellulase |
| Тема | Trichoderma viride |
| Тема | glutaraldehyde |
| Тема | IgG‐Px conjugate |
| Тема | peroxidase |
| Название | An enzyme‐linked immunosorbent assay for cellulase (from trichoderma viride) in animal feed |
| Тип | research-article |
| DOI | 10.1080/09540109109354726 |
| Electronic ISSN | 1465-3443 |
| Print ISSN | 0954-0105 |
| Журнал | Food and Agricultural Immunology |
| Том | 3 |
| Первая страница | 21 |
| Последняя страница | 27 |
| Аффилиация | Burianová, T.; Institute of Chemical Technology |
| Аффилиация | Sajdok, J.; Institute of Chemical Technology |
| Аффилиация | Káš, J.; Institute of Chemical Technology |
| Выпуск | 1 |
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