Автор |
Rungkat‐Zakaria, Fransiska |
Автор |
Belleville, Francine |
Автор |
Nabet, Pierre |
Автор |
Linden, Guy |
Дата выпуска |
1992 |
dc.description |
First generations of milk protein exempted mice (6–8 weeks old) were fed with casein at 100 mg per day. At 0, 2, 4, 6, 8, 12 and 20 weeks of feeding, a group of eight mice was sacrificed to recover spleen lymphocytes and intraperitoneal mastocytes. In vitro lymphocyte <sup>3</sup>H‐thymidine incorporation in the presence of intact casein, peptic or peptic‐tryptic‐chymotryptic hydrolysate was significantly higher at eight and 12 weeks of feeding (p<0.05). The number of the sensitized mice increased from 39% in the control group to 61% in the experimental groups. The three antigens possessed a statistically equal capacity to stimulate lymphocyte proliferation. Measurements of plasma and culture supernatant IgG antibody production exhibited an elevated enzyme‐linked immunosorbent assay value starting from the eight week of casein feeding. Casein feeding also provoked histamine accumulation in the mastocytes which occured significantly after four weeks of feeding (p<0.001). The fact that the lymphocytes recognized not only intact casein but also its digestive enzyme hydrolyzates indicate that intestinal digestion and absorption processes could neither totally block the antigen passage nor destroy their epitopes. |
Формат |
application.pdf |
Издатель |
Taylor & Francis Group |
Копирайт |
Copyright Taylor and Francis Group, LLC |
Тема |
antigen |
Тема |
casein |
Тема |
histamine |
Тема |
lymphocyte |
Тема |
mastocytes |
Тема |
proliferation |
Название |
Allergenicity of bovine casein. II: Casein and Its digestive enzyme hydrolysate‐induced lymphocyte proliferation and Mastocyte histamine accumulation in casein‐free mice |
Тип |
research-article |
DOI |
10.1080/09540109209354752 |
Electronic ISSN |
1465-3443 |
Print ISSN |
0954-0105 |
Журнал |
Food and Agricultural Immunology |
Том |
4 |
Первая страница |
51 |
Последняя страница |
62 |
Аффилиация |
Rungkat‐Zakaria, Fransiska; Laboratory of Applied Biochemistry associated with INRA, University of Nancy I; Laboratory of Medical Biochemistry I, University of Nancy I and IBN; Laboratoire de Biochimie Appliquée, Université de Nancy I |
Аффилиация |
Belleville, Francine; Laboratory of Medical Biochemistry I, University of Nancy I and IBN |
Аффилиация |
Nabet, Pierre; Laboratory of Medical Biochemistry I, University of Nancy I and IBN |
Аффилиация |
Linden, Guy; Laboratory of Applied Biochemistry associated with INRA, University of Nancy I |
Выпуск |
1 |
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