| Автор | Dürsch, Inge |
| Автор | Meyer, Heinrich H. D. |
| Дата выпуска | 1992 |
| dc.description | A multi‐residue screening test was developed which provides reliable detection of eight ß‐agonists in urine, based on an enzyme immunoassay with peroxidase and an antibody raised against clenbuterol‐diazo‐BSA. Antibodies were compared after continuous immunization. The antibody obtained after the 12th immunization showed the best sensitivity, procedural blanks of urine samples and coefficients of variation, using analysis of spiked urine samples. The simultaneous clean‐up was performed with silicagel RP select‐B. In 125 samples from untreated female or male cattle or pigs, sample blanks gave results of <0·07 ng clenbuterol equivalents/ml urine and in two further samples, results of 0·13 and 0·15 ng/ml, respectively. The method showed coefficients of variation of 20–30% for the ß‐agonists tested, except in the case of orciprenaline, at levels ranging from 0·1 ng/ml urine (clenbuterol, mabuterol) to 10 ng/ml urine (pirbuterol, orciprenaline). These ß‐agonists were also separated by a high performance liquid chromatography (HPLC) system, which provided complete separation of orciprenaline, 2‐amino‐3‐chloro‐5‐(1'‐hydroxy‐2'amino‐tert‐butyl‐ethyl) pyridine (ACP), isoetharine, clenbuterol and mabuterol as well as good separation of carbuterol, terbutaline, salbutamol and pirbuterol. |
| Формат | application.pdf |
| Издатель | Taylor & Francis Group |
| Копирайт | Copyright Taylor and Francis Group, LLC |
| Тема | Antibody |
| Тема | ß‐agonists |
| Тема | EIA |
| Тема | HPLC |
| Тема | multi‐residue screening |
| Название | A multi‐residue enzyme immunoassay for screening illegally used β‐agonists |
| Тип | research-article |
| DOI | 10.1080/09540109209354770 |
| Electronic ISSN | 1465-3443 |
| Print ISSN | 0954-0105 |
| Журнал | Food and Agricultural Immunology |
| Том | 4 |
| Первая страница | 211 |
| Последняя страница | 218 |
| Аффилиация | Dürsch, Inge; Institüt für Physiologie des Forschungszentrums für Milch und Lebensmittel, Weihenstephan, Technische Universität München‐Weihenstephan |
| Аффилиация | Meyer, Heinrich H. D.; Institüt für Physiologie des Forschungszentrums für Milch und Lebensmittel, Weihenstephan, Technische Universität München‐Weihenstephan |
| Выпуск | 4 |
| Библиографическая ссылка | Baker, P. K., Dalrymple, R. H., Ingle, D. L. and Ricks, C. A. 1984. Use of a β‐adrenergic agonist to alter muscle and fat deposition in lambs. Journal of Animal Science, 59: 1256–1261. |
| Библиографическая ссылка | Dalrymple, R. H., Baker, P. K. and Ricks, C. A. 1984. Repartitioning agents to improve performance and body composition. Proceedings of the Georgia Nutrition Conference, : 111–118. |
| Библиографическая ссылка | Harlow, E. and Lane, D. 1988. Antibodies: a laboratory manual, New York, , USA: Cold Spring Harbor Laboratory. |
| Библиографическая ссылка | Meyer, H. H. D. 1991. The illegal practice and resulting risks versus the controlled use of licensed drugs: views on the present situation in Germany. Annales de Recherches Vétérinaires, 22: 299–304. |
| Библиографическая ссылка | Meyer, H. H. D. and Rinke, L. 1991. The pharmakokinetics and residues of clenbuterol in veal calves. Journal of Animal Science, 69: 4538–4544. |
| Библиографическая ссылка | Meyer, H. H. D., Rinke, L. and Dürsch, I. 1991. Residue screening for the β‐agonists clenbuterol, salbutamol and cimaterol in urine using enzyme immunoassay and high performance liquid chromatography. Journal of Chromatography, 564: 551–556. |
| Библиографическая ссылка | Ricks, C. A., Dalrymple, R. H., Baker, P. K. and Ingle, D. L. 1984. Use of β‐agonist to alter fat and muscle deposition in steers. Journal of Animal Science, 59: 1247–1255. |
| Библиографическая ссылка | Van Ginkel, L. A., Stephany, R. W., Rossum, H. J. van and Farla, J. 1990. “Multiresidue test for β‐agonists in slaughter animals”. In International symposium on ‘New trends in veal calf production’, Wageningen, , The Netherlands: International Agricultural Centre. |
| Библиографическая ссылка | Yamamoto, I. and Iwata, K. 1982. Enzyme immunosassay for clenbuterol, a β<sub>2</sub>‐adrenergic stimulant. Journal of Immunoassay, 2: 155–171. |
