Мобильная версия

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Автор Karube, Isao
Автор Tamiya, Eiichi
Дата выпуска 1987
dc.description AbstractNovel biosensor systems were constructed for food industry. In order to determine fish freshness, a multifunctional enzyme sensor system was developed by combining a double membrane consisting of a 5'‐nucleotidase membrane and a nucleoside phosphorylase‐xanthine oxidase membrane with an oxygen electrode. Each nucleotide concentration was determined as the current decreased. One assay was completed within 20 min. Good comparative results were observed between the KI values determined by the sensor proposed and by the conventional method. An enzyme sensor for meat freshness consisted of a monoamine oxidase‐collagen membrane and an oxygen electrode. The response time of the electrode was 4 min. A linear relationship was observed between the amine (tyramine) concentration in the range 50–200 μM and the difference in current. Monoamine in meat extract was determined by the enzyme sensor. Micro‐glutamate sensor was constructed using silicon fabrication technology. Calibration curve for glutamate sensor was obtained in a glutamate concentration range between 5–50 mM. Moreover, an immuno sensor based on piezoelectric crystal was applied to the determination of toxic bacterium C. albicans. The frequency shift is correlated with C. albicans concentration in the range 10<sup>6</sup> 5×10<sup>8</sup> cell/ml.
Формат application.pdf
Издатель Taylor & Francis Group
Копирайт Copyright Taylor and Francis Group, LLC
Название Biosensors fob food industry
Тип research-article
DOI 10.1080/08905438709549665
Electronic ISSN 1532-4249
Print ISSN 0890-5436
Журнал Food Biotechnology
Том 1
Первая страница 147
Последняя страница 165
Аффилиация Karube, Isao; Research Laboratory of Resources Utilization, Tokyo Institute of Technology
Аффилиация Tamiya, Eiichi; Research Laboratory of Resources Utilization, Tokyo Institute of Technology
Выпуск 2
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Библиографическая ссылка Karube, I., Satoh, I., Araki, Y. and Suzuki, S. 1980. Monoamine oxidase electrode in freshness testing of meat. Enzyme Microb Technol., 2: 117–120.
Библиографическая ссылка Karube, I and Suzuki, S. 1984. “Amperometric and potentiometric determination with immobilized enzymes and microorganisms”. In Ion‐Selective Electrode Reviews, Vol.6, 15–58. Pergamon Press.
Библиографическая ссылка Karube, I., Matsuoka, H., Suzuki, S., Watanabe, K. and Toyama, K. 1984. Determination of fish freshness with an enzyme sensor system. J Agric Food Chem., 32: 314–319.
Библиографическая ссылка Karube, I and Suzuki, S. 1985. “Immobilized enzymes for clinical analysis”. In Enzymes and immobilized cells in biotechnology, 209–226. Benjamin/Cumming Publishing.
Библиографическая ссылка Karube, I. 1987. “Micro‐biosensors based on silicon fabrication technology”. In BIOSENSORS ‐Fundamentals and Applications, 471–480. Oxford University Press.
Библиографическая ссылка Muramatsu, H., Kajiwara, K., Tamiya, E. and Karube, I. 1986. Piezoelectric immuno sensor for the detection of Candida albicans microbes. Anal Chim.. Acta, 188: 257–261.
Библиографическая ссылка Roederer, J.E. and Bastiaans, G.J. 1983. Microgravimetric immunoassay with piezoelectric crystals. Anal Chem., 55: 2333–2336.
Библиографическая ссылка Watanabe, E., Ando, K., Karube, I., Matsuoka, H. and Suzuki, S. 1983. Determination of hypoxanthine in fish meat with an enzyme sensor. J Food Sci., 48: 496–500.
Библиографическая ссылка Watanabe, E., Toyama, K., Karube, I., Matsuoka, H. and Suzuki, S. 1984. Determination of inosine‐5‐monophosphate in fish tissue with an enzyme sensor. J Food Sci., 49: 114–116.

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