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Автор Sudarshan, N.R.
Автор Hoover, D.G.
Автор Knorr, D.
Дата выпуска 1992
dc.description AbstractThe antibacterial action of chitosan hydroglutamate (CH), chitosan lactate (CL) and chitosan derived from fungal mycelia was examined against both gram‐negative and gram‐positive bacteria. Plate counts indicated inactivation rates of one‐ to five‐log‐cycles within one hour. Fungal chitosan had significantly less antibiotic effect than CH and CL. The antibacterial action of CH and CL was very similar and shown to be concentration dependent with 0.1 mg/mL more effective than 2.0 and 5.0 mg/mL. When CH (or CL) and polygalacturonate were added to cell suspensions, death was prevented, possibly indicating that chitosan complexed with polygalacturonate could not penetrate the cell or disrupt the membrane. Leakage of intracellular components caused by chitosan was determined by exposing lactose‐induced Escherichia coli to chitosan with assay for ß‐galactosidase activity indicating that cell permeabilization occurred more extensively at the low chitosan concentrations. Microscopic examination showed that chitosan caused cell agglutination at pH 5.8. Injury to chitosan‐exposed Staphylococcus aureus MF‐31 could not be demonstrated using the criterion that sublethally stressed cells have increased sensitivity to high levels of sodium chloride.
Формат application.pdf
Издатель Taylor & Francis Group
Копирайт Copyright Taylor and Francis Group, LLC
Название Antibacterial action of chitosan
Тип research-article
DOI 10.1080/08905439209549838
Electronic ISSN 1532-4249
Print ISSN 0890-5436
Журнал Food Biotechnology
Том 6
Первая страница 257
Последняя страница 272
Аффилиация Sudarshan, N.R.; Department of Food Science, University of Delaware; Department of Food Science and Technology, Oregon State University
Аффилиация Hoover, D.G.; Department of Food Science, University of Delaware
Аффилиация Knorr, D.; Department of Food Science, University of Delaware; Department of Food Technology, Berlin University of Technology
Выпуск 3
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