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Автор Hall, M.
Автор Eldridge, D. B.
Автор Saunders, R. D.
Автор Fairclough, D. L.
Автор Bateman, R. C.
Дата выпуска 1995
dc.description AbstractThis study describes the production of a solid phase assay (dipstick test) for histamine in tuna based on the coupling of diamine oxidase to a peroxidase/dye system. The optimized dipstick preparation consisted of a diamine oxidase/peroxidase/Stabilcoat mixture layered onto a tetramethylbenzidine color strip. The assay was linear to 1.0 mM histamine (RSQ = 0.994) and the minimum detectable concentration was 0.07 mM corresponding to 2.3 mg% in tuna extracts. Quality control samples gave intra and interassay precisions of 9.14–9.84 %CV and 7.08–13.86 %CV respectively. Similarly, the assay was accurate (p> 0.05) for both low and high controls. Histidine and cadaverine did not interfere with the assay whereas putrescine reacted slightly with the dipstick. Histamine determinations of fresh and spoiled tuna gave good agreement between the dipstick method and both the official reference and modified AOAC flurometric methods.
Формат application.pdf
Издатель Taylor & Francis Group
Копирайт Copyright Taylor and Francis Group, LLC
Название A rapid dipstick test for histamine in tuna
Тип research-article
DOI 10.1080/08905439509549884
Electronic ISSN 1532-4249
Print ISSN 0890-5436
Журнал Food Biotechnology
Том 9
Первая страница 39
Последняя страница 57
Аффилиация Hall, M.; Department of Medical Technology, Univ of Southern Mississippi
Аффилиация Eldridge, D. B.; Department of Chemistry and Biochemistry, Univ of Southern Mississippi
Аффилиация Saunders, R. D.; Department of Chemistry and Biochemistry, Univ of Southern Mississippi
Аффилиация Fairclough, D. L.; Department of Biostatistics, Dana Farber Cancer Institute
Аффилиация Bateman, R. C.; Department of Chemistry and Biochemistry, Univ of Southern Mississippi
Выпуск 1-2
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