| Автор | Michaelsen, Søren |
| Автор | Otte, Jeanette |
| Автор | Simonsen, Lars-Ole |
| Автор | Sørensen, Hilmer |
| Дата выпуска | 1994 |
| dc.description | AbstractDegradation of intact glucosinolates or their transformation into other products and glucosinolate absorption from the gastrointestinal tract were investigated by in vivo and in vitro experiments with rats. The absorption of intact glucosinolates in the form of active and passive transport across the intestinal wall was investigated in vitro using the intestine of rat and hamster as everted sacs. Individual glucosinolates as well as rapeseed meal with and without heat inactivation of myrosinase activity were used for the experiments. Analyses were based on HPLC and HPCE after group separation, purification and concentration of intact glucosinolates and their on-column desulfatation products. The in vivo experiments showed 3- to 20-fold lower concentrations of glucosinolates in the content from the stomach and the first parts of the small intestine compared to the concentrations in the applied feed. The glucosinolate concentrations increased again in contents from the distal part of the small intestine, and were reduced to very low values in contents from caecum and colon, with great variations between the individual animals. In vitro studies of glucosinolate degradation during 24 h periods revealed limited degradations when tissue homogenates of the stomach and small intestine and contents therein were used, whereas appreciable degradations were found when the caecum and/or colon contents were used. Heat inactivation of myrosinase activity in the rapeseed meal fed to a group of rats did not affect in vitro glucosinolate degradation in contents from stomach or small intestine, whereas the degradation seemed to be prevented in contents from caecum or colon, indicating an effect from dietary fibres or myrosinase associated to the dietary fibres. None of the glucosinolates was absorbed actively across the intestinal walls in vitro for any parts of the gastrointestinal tract investigated, as found with glucose, whereas passive or facilitated transport was found to be appreciable. The presence of glucosinolates did not affect the active glucose transport. The results obtained showed an appreciable in vivo absorption of intact glucosinolates in the upper gastrointestinal tract and degradation/transformation of the remaining glucosinolates in the distal gastrointestinal tract of rats. |
| Формат | application.pdf |
| Издатель | Taylor & Francis Group |
| Копирайт | Copyright Taylor and Francis Group, LLC |
| Тема | intestinal contents |
| Тема | intestinal wall |
| Тема | everted sacs |
| Тема | active transport |
| Тема | facilitated transport |
| Тема | passive transport |
| Тема | rapeseed |
| Тема | dietary fibres |
| Тема | myrosinases |
| Название | Absorption and Degradation of Individual Intact Glucosinolates in the Digestive Tract of Rodents |
| Тип | research-article |
| DOI | 10.1080/09064709409410178 |
| Electronic ISSN | 1651-1972 |
| Print ISSN | 0906-4702 |
| Журнал | Acta Agriculturae Scandinavica, Section A – Animal Science |
| Том | 44 |
| Первая страница | 25 |
| Последняя страница | 37 |
| Аффилиация | Michaelsen, Søren; Chemistry Department, Royal Veterinary and Agricultural University |
| Аффилиация | Otte, Jeanette; Chemistry Department, Royal Veterinary and Agricultural University |
| Аффилиация | Simonsen, Lars-Ole; Zoophysiological Laboratory B, August Krogh Institute |
| Аффилиация | Sørensen, Hilmer; Chemistry Department, Royal Veterinary and Agricultural University |
| Выпуск | 1 |
| Библиографическая ссылка | Alvarado, F. and Crane, R. K. 1964. Studies of the mechanism of intestinal absorption of sugars. VII. Phenylglucoside transport and its possible relationship to phlorizin inhibition of the active transport of sugars by the small intestine. Biochim. Biophys. Acta, 93: 116–135. |
| Библиографическая ссылка | Bell, J. M., Keith, M. O. and Hutcheson, D. S. 1991. Nutritional evaluation of very low glucosinolate canola meal. Can. J. Anim. Sci., 71: 497–506. |
| Библиографическая ссылка | Bille, N., Eggum, B. O., Jacobsen, I., Olsen, O. and Sørensen, H. 1983(a). The effects of processing on antinutritional rape constituents and the nutritive value of double low rapeseed meal. 2. Tierphysiol. Tierernährung u. Futtermittelkd., 49: 148–163. |
| Библиографическая ссылка | Bille, N., Eggum, B. O., Jacobsen, I., Olsen, O. and Sørensen, H. 1983(b). Antinutritional and toxic effects in rats of individual glucosinolates (± myrosinases) added to a standard diet. 1. The effects on protein utilization and organ weights. 2. Tierphysiol. Tierernährung u. Futtermittelkd., 49: 195–210. |
| Библиографическая ссылка | Bjerg, B. and Sørensen, H. 1987(a). “Isolation of intact glucosinolates by column chromatography and determination of their purity”. In World Crops: Production, Utilization, Description. Glucosinolates in Rapeseeds: Analytical Aspects, Edited by: Wathelet, J.-P. 59–75. Dordrecht / Boston / Lancaster: Martinus Nijhoff Publ., Kluwer Academic Publishers Group. |
| Библиографическая ссылка | Bjerg, B. and Sørensen, H. 1987(b). “Quantitative analysis of glucosinolates in oilseed rape based on HPLC of desulfoglucosinolates and HPLC of intact glucosinolates”. In World-Crops: Production, Utilization, Description. Glucosinolates in Rapeseeds: Analytica Aspects, Edited by: Wathelet, J.-P. Vol. 13, 125–150. Dordrecht / Boston / Lancaster: Martinus Nijhoff Publ., Kluwer Academic Publishers Group. |
| Библиографическая ссылка | Bjerg, B., Eggum, B. O., Jacobsen, I., Otte, J. and Sørensen, H. 1989. Antinutritional and toxic effects in rats of individual glucosinolates (±myrosinases) added to a standard diet (2). Z. Tierphysiol. Tierernährung u. Futtermittelkd., 61: 227–244. |
| Библиографическая ссылка | Bjergegaard, C., Eggum, B. O., Jensen, S. K. and Sørensen, H. 1991. Dietary fibres in oilseed rape: Physiological and antinutritional effects in rats of isolated IDF and SDF added to a standard diet (1). J. Anim. Physiol. Anim. Nutr., 66: 69–79. |
| Библиографическая ссылка | Björkman, R. 1973. Interaction between proteins and glucosinolate isothiocyanates and oxazolidinthiones from Brassica napus seed. Phytochemistry, 12: 1585–1590. |
| Библиографическая ссылка | Bowland, J. P. 1974. Comparison of low glucosinolate rapeseed meal, commercial rapeseed meal and soybean meal as protein supplements for growing pigs. Can. J. Anim. Sci., 54: 679–685. |
| Библиографическая ссылка | Chung, F.-L., Wang, M. and Hecht, S. S. 1985. Effects of dietary indoles and isothiocyanates on N-nitrosodimethylamine and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone α-hydroxylation and DNA methylation in rat liver. Carcinogenesis, 6: 539–543. |
| Библиографическая ссылка | Danielsen, V., Eggum, B. O., Rasmussen, K. W. and Sørensen, H. Long-term studies of requirements to the quality of rapeseed meal from double low varieties used in sow diets. 7th International Rapeseed Congress, Poznan, Poland, V. Vol. 7, pp.1727–1734. |
| Библиографическая ссылка | Darroch, C. S., Bell, J. M., McGregor, D. I. and Mills, J. H. L. 1991. The effects of a linear increase in dietary indole glucosinolates on growth and physiology of mice. Can. J. Anim. Sci., 71: 887–896. |
| Библиографическая ссылка | Dashwood, R. H., Arbogast, D. N., Fong, A. T., Pereira, C., Hendricks, J. D. and Bailey, G. S. 1989. Quantitative interrelationships between aflatoxin B<sub>1</sub> carcinogen dose, indole-1-carbinol anti-carcinogen dose, target organ DNA adduction and final tumor response. Carcinogenesis, 10: 175–181. |
| Библиографическая ссылка | Eggum, B. O. 1981. “Nutritional problems related to double low rapeseed in animal nutrition”. In Production and utilization of protein and oilseed crops, Edited by: Bunting, E. S. 293–310. Dordrecht / Buston / Lancaster: Martinus Nijhoff Publ.. |
| Библиографическая ссылка | Eggum, B. O., Olsen, O. and Sørensen, H. 1985. “Effects of glucosinolates on the nutritive value of rapeseed”. In Advances in the production and utilization of cruciferous crops, Edited by: Sørensen, H. Vol. 11, 50–60. Dordrecht / Boston / Lancaster: Martinus Nijhoff / Dr. W. Junk Publ.. |
| Библиографическая ссылка | Freig, A. A. H., Campbell, L. D. and Stanger, N. E. 1987. Fate of ingested glucosinolates in poultry. Nutr. Rep. Internat., 36: 1337–1345. |
| Библиографическая ссылка | Greer, M. A. 1962. The natural occurence of goitrogenic agents. Rec. Prog. Horm. Res., 18: 187–219. |
| Библиографическая ссылка | Henriksen, P., Hillemann, G., Mortensen, K. and Sorensen, H. Requirements to the quality of oilseed rape which can be used without problems in diets to mink (Mustela vison Screb.). 7th International Rapeseed Congress, Poznan, Poland, VII. vol. 7, pp.1817–1824. |
| Библиографическая ссылка | Von Jahreis, G., Hesse, V., Schöne, F., Lüdke, H., Hennig, A. and Mehnert, E. 1986. Einfluß von Nitrat und pflanzlichen Goitrogenen auf die Schilddrüsenhormone, den Somatome-dinstatus und das Wachstum beim Schwein. Monatsh. Veteri-närmed., 41: 528–530. |
| Библиографическая ссылка | Jensen, S. K., Olsen, H. S. and Sørensen, H. 1990. “Aqueous enzymatic processing of rapeseed for production of high quality products”. In Canola and Rapeseed: Production, Chemistry, Nutrition and Processing Technology, Edited by: Shahidi, F. 331–343. Van Nostrand Reinhold. chapt. 19 |
| Библиографическая ссылка | Jensen, S. K., Michaelsen, S., Kachlicki, P. and Sorensen, H. 1991. “4-Hydroxyglucobrassicin and degradation products of glucosinolates in relation to unsolved problems with the quality of double low oilseed rape”. In GCIRC Congress Vol. V, 1359–1364. Saskatoon, , Canada |
| Библиографическая ссылка | Landau, B. R., Bernstein, L. and Wilson, T. H. 1962. Hexose transport by hamster intestine in vitro. Am. J. Physiol., 203: 237–240. |
| Библиографическая ссылка | Lo, M. T. and Bell, J. M. 1972. Effects of various dietary glucosinolates on growth, feed intake and thyroid function in rats. Can. J. Anim. Sci., 52: 295–302. |
| Библиографическая ссылка | Lo, M. T. and Hill, D. C. 1971. Toxicity of a glucosinolate concentrate prepared from rapeseed meal. Can. J. Anim. Sci., 51: 197–192. |
| Библиографическая ссылка | Lo, M. T. and Hill, D. C. 1972. Glucosinolates and their hydrolytic products in intestinal contents, feces, blood, and urine of rats dosed with rapeseed meals. Can. J. Physiol. Pharmacol., 50: 962–966. |
| Библиографическая ссылка | Loft, S., Otte, J., Poulsen, H. E. and Sorensen, H. 1992. Influence of intact and myrosinase-treated indolyl glucosinolates on the metabolism in vivo of metronidazole and antipyrine in the rat. Food Chem. Toxic., 30: 927–935. |
| Библиографическая ссылка | Marangos, A. and Hill, R. 1974. The hydrolysis and absorption of thioglucosides and rapeseed meal. Proc. Nutr. Soc., 73: 90A |
| Библиографическая ссылка | Michaelsen, S., Mortensen, K. and Sorensen, H. 1991a. “Myrosinases in Brassica: Characterization and properties”. In GCIRC-Congress Vol. III, 905–910. Saskatoon, , Canada |
| Библиографическая ссылка | Michaelsen, S., Mortensen, K. and Sørensen, H. 1991b. “Heat and microwave processing of oilseed rape: Effects on product quality”. In CGIRC-Congress Vol. VI, 1872–1878. Saskatoon, , Canada |
| Библиографическая ссылка | Michaelsen, S., Møller, P. and Sørensen, H. 1991(c). High performance capillary electrophoresis: A fast, cheap and simple method of analysis for determination of individual glucosinolates. Bulletin-GCIRC, 7: 97–105. |
| Библиографическая ссылка | Nishie, K. and Daxenbichler, M. E. 1980. Toxicology of glucosinolates, related compounds (nitriles, R-goitrin, isothiocyantes) and vitamin U found in Cruciferae. Food Cosmet. Toxicol., 18: 159–172. |
| Библиографическая ссылка | Nugon-Baudon, L., Szylit, O. and Raibaud, P. 1988. Production of toxid glucosinolate derivatives from rapeseed meal by intestinal microflora of rat and chicken. J. Sci. Food Agric., 43: 299–308. |
| Библиографическая ссылка | Nugon-Baudon, L., Rabot, S., Wal, J.-M. and Szylit, O. 1990(a). Interactions of the intestinal microflora with glucosinolates in rapeseed meal toxicity: First evidence of an intestinal lacto-bacillus possessing a myrosinase-like activity in vitro. . J. Sci. Food Agric., 52: 547–559. |
| Библиографическая ссылка | Nugon-Baudon, L., Rabot, S., Szylit, O. and Raibaud, P. 1990(b). Glucosinolates toxicity in growing rats: interactions with the hepatic detoxification system. Xenobiotica, 20: 223–230. |
| Библиографическая ссылка | Oginsky, E. L., Stein, A. E. and Greer, M. A. 1965. Myrosinase activity in bacteria as demonstrated by the conversion of progoitrin to goitrin. Proc. Soc. Exp. Biol. Med., 119: 360–364. |
| Библиографическая ссылка | Olsen, O. and Sorensen, H. 1981. Recent advances in the analysis of glucosinolates. J. Am. Oil Chem. Soc., 58: 857–865. |
| Библиографическая ссылка | Searle, L. M., Chamberlain, K. and Butcher, D. N. 1984. Preliminary studies on the effects of copper, iron and manganese ions and the degradation of 3-indolylmethylglucosinolate (a constituent of Brassica spp.) by myrosinase. J. Sci. Food Agric., 35: 745–748. |
| Библиографическая ссылка | Slominski, B. A., Campbell, L. D. and Stanger, N. E. 1987. Influence of cecectomy and dietary antibiotics on the fate of incested intact glucosinolates in poultry. Can. J. Anim. Sci., 67: 1117–1124. |
| Библиографическая ссылка | Slominski, B. A., Campbell, L. D. and Stanger, N. E. 1988. Extent of hydrolysis in the intestinal tract and potential absorption of intact glucosinolates in laying hens. J. Sci. Food Agric., 42: 305–314. |
| Библиографическая ссылка | Somogyi, M. 1945. Determination of blood susar. J. Biol. Chem., 160: 69–73. |
| Библиографическая ссылка | Sorensen, H. 1990. “Glucosinolates: Structure-Properties-Function”. In Canola and Rapeseed: Production, Chemistry, Nutrition and Processing Technology, Edited by: Shahidi, F. 149–172. Van Nos-trand Reinhold. chapt. 9 |
| Библиографическая ссылка | Vermorel, M., Heaney, R. K. and Fenwick, G. R. 1986. Nutritive value of rapeseed meal: Effects of individual glucosinolates. J. Sci. Food Agric., 37: 1197–1202. |
| Библиографическая ссылка | Wattenberg, L. W., Hanley, A. B., Barany, G., Sparnins, V. L., Lam, L. K. T. and Fenwick, R. G. 1986. “Inhibition of carcinogenesis by some minor dietary constituents”. In Diet, Nutrition and Cancer, Edited by: Hayashi, Y. 193–203. Utrecht: Japan Sci. Soc. Press, Tokyo/VNU Sci. Press. |
| Библиографическая ссылка | Wattenberg, L. W. and Loub, W. D. 1978. Inhibition of polycyclic aromatic hydrocarbon-induced neoplasia by naturally occuring indoles. Cancer Res., 38: 1410–1413. |
| Библиографическая ссылка | Wilson, T. H. 1962. Intestinal absorption, Philadelphia: W. B. Saunders Company. |
