| Автор | Kalén, Gunnar |
| Автор | Samuelsson, Robert |
| Автор | Törmä, Eva |
| Дата выпуска | 1992 |
| dc.description | AbstractA method for the determination of hordenine, gramine, 5-methoxy-N,N-dimethyltryptamine and N-methyltryptamine in reed canarygrass is described. The material is chopped into pieces, 0.5–1 cm, and extracted overnight in 20% ethanol and 1% acetic acid. The extract is purified by solid phase extraction using a strong cation exchange column. Interferences are removed by selective elution with water and methanol. The alkaloids are eluted with 1 M potassium acetate in methanol and the analysis is carried out by HPLC and UV detection. The column used was Kromasil C<sub>18</sub> and the eluent comprised 30% methanol, 1 mM N,N-dimethyloctylamine, 10 mM sodium heptansulfonic acid and 25 mM citrate buffer, pH 3.1. The influence of eluent composition, together with extraction and purification parameters, was investigated. The alkaloids recovered by the extraction and determination methods ranged from 74 to 85% and the alkaloid concentration determined in the variety Palaton was in good agreement with earlier reports. |
| Формат | application.pdf |
| Издатель | Taylor & Francis Group |
| Копирайт | Copyright Taylor and Francis Group, LLC |
| Тема | gramine |
| Тема | hordenine |
| Тема | 5-methoxy-N,N-dimethyltryptamine |
| Тема | N-methyltryptamine |
| Название | A Method for the Determination of Alkaloids in Reed Canarygrass (Phalaris arundinaceae L.) using HPLC and Solid Phase Extraction |
| Тип | research-article |
| DOI | 10.1080/09064719209410216 |
| Electronic ISSN | 1651-1913 |
| Print ISSN | 0906-4710 |
| Журнал | Acta Agriculturae Scandinavica, Section B – Soil & Plant Science |
| Том | 42 |
| Первая страница | 224 |
| Последняя страница | 229 |
| Аффилиация | Kalén, Gunnar; National Laboratory for Agricultural Chemistry |
| Аффилиация | Samuelsson, Robert; National Laboratory for Agricultural Chemistry |
| Аффилиация | Törmä, Eva; National Laboratory for Agricultural Chemistry |
| Выпуск | 4 |
| Библиографическая ссылка | Duynisveld, G. W., Slominski, B. A., Wittenberg, K. M. and Campbell, L. D. 1990. Alkaloid content of reed canarygrass (Phalaris arundinaceae L.) as determined by gas-liquid chromatography. Can. J. Plant Sci., 70: 1097–1103. |
| Библиографическая ссылка | Hagman, J. L., Marten, G. C. and Hovin, A. W. 1975. Alkaloid concentration in plant parts in reed canarygrass of varying maturity. Crop Sci., 15: 41–43. |
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| Библиографическая ссылка | Majak, W., McDiarmidet, R. E., Powell, T. W., Van Ryswyk, A. L., Stout, D. G., Williams, R. J. and Tucker, R. E. 1979. Relationship between alkaloids in reed canarygrass. (Phalaris arundinaceae), soil moisture and nitrogen fertility. Plant Cell Environ., 2: 335–340. |
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| Библиографическая ссылка | Marten, G. C., Jordan, R. M. and Hovin, A. W. 1981. Improved lamb performance associated with breeding for alkaloid reduction in reed canarygrass. Crop Sci., 21: 295–298. |
| Библиографическая ссылка | Olsson, R., Sandström, T., Dahlgren, O. and Dahlgren, L. 1989. Sameldning energigräs torv, miljö-ekonomi, Uppsala, , Sweden: Swedish University of Agricultural Sciences. SLL rapport nr. 63 |
| Библиографическая ссылка | Östrem, L. 1987. Studies on genetic variation in reed canarygrass, Phalaris arundinacea L. Hereditas, 107: 235–248. |
| Библиографическая ссылка | Simons, A. B. and Marten, G. C. 1971. Relationship of indole alkaloids to palatibility of Phalaris arundinacea L. Agron. J., 63: 915–919. |
| Библиографическая ссылка | Woods, D. L., Hovin, A. W. and Marten, G. C. 1979. Seasonal variation of hordenine and gramine concentrations and their heritability in reed canarygrass. Crop Sci., 19: 853–857. |
