Isolation and regeneration of protoplasts from Porphyra dentata and Porphyra crispata
Gall (Yvan Le Gall), Erwan Ar; Chiang, Young-Meng; Kloareg, Bernard; Gall (Yvan Le Gall), Erwan Ar; Institute of Oceanography, National Taiwan University; Centre d'Etudes Océanographiques et de Biologie Marine, CNRS (UPR 4601) and Université Paris VI; Chiang, Young-Meng; Institute of Oceanography, National Taiwan University; Kloareg, Bernard; Institute of Oceanography, National Taiwan University; Centre d'Etudes Océanographiques et de Biologie Marine, CNRS (UPR 4601) and Université Paris VI
Журнал:
European Journal of Phycology
Дата:
1993
Аннотация:
Large numbers of protoplasts (10<sup>6</sup> to 3·2 × 10<sup>7</sup> per gram fresh weight) were routinely isolated from two Taiwanese species of Porphyra: P. dentata and P. crispata. Intermediate and final forms of regeneration were observed, including swollen cells, monospore-like cells, filaments, callus-like structures and bladelets. Regeneration of leafy plants was achieved from protoplasts of P. crispata, whereas only conchocelis-like filaments were regenerated from protoplasts of P. dentata. In this latter species, conchosporangia and monosporangia appeared on the filaments upon cultivation at 25 °C. The variability in regenerative patterns is discussed.
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