| Автор | Medina, Marjorie B. |
| Автор | Schwartz, Daniel P. |
| Дата выпуска | 1987 |
| dc.description | A multi‐residue HETLC (High Efficiency Thin Layer Chromatography) screening procedure for 17β‐oestradiol, diethylstilboesterol (DES), zearalanol (zeranol), zearalenone and their metabolites oestrone, zearalanone, and zearalenol is described. The anabolic oestrogens were analyzed on HETLC plates coated with silica gel and were developed in methylene chloride:methanol: 2‐propanol (97:1:2 v/v). The spots were visualized by exposure to iodine vapours and subsequently sprayed with 1% starch solution. Analysis of standards by HETLC at 4°C as a seven‐component mixture showed six discrete bands with mean R<sub>t</sub>s of 0.37 (oestrone), 0.35 (zearalanone and zearalenone), 0.26 (t‐DES), 0.23 (oestradiol), 0.17 (zearalenol and zearalanol), and 0.15 (c‐DES). Chicken muscle tissues (1, 2.5, or 5g) were extracted with 95% acetone. Extracts were then fortified with 50–250 ng each of the anabolic oestrogens, purified in alumina and ion‐exchange columns and analyzed by HETLC. Oestradiol, zeranol or DES in fortified tissue extracts were clearly detected when an equivalent of 4ng were analyzed by HETLC after purification in alumina and ion‐exchange columns. The intensity of their bands suggested near quantitative recovery when compared to intensity of bands of known amounts of standards. The described extraction, purification, and TLC procedures can be used to screen these oestrogens at low ppb amounts in chicken muscle tissues and should be applicable to screen tissues of cattle and sheep. |
| Формат | application.pdf |
| Издатель | Taylor & Francis Group |
| Копирайт | Copyright Taylor and Francis Group, LLC |
| Название | A multi‐residue TLC screening procedure for anabolic oestrogens and detection of oestradiol, DES or zeranol in chicken muscle tissue extracts |
| Тип | research-article |
| DOI | 10.1080/02652038709373650 |
| Print ISSN | 0265-203X |
| Журнал | Food Additives and Contaminants |
| Том | 4 |
| Первая страница | 415 |
| Последняя страница | 427 |
| Аффилиация | Medina, Marjorie B.; Agriculture Research Service, U.S. Department of Agriculture, Eastern Regional Research Center |
| Аффилиация | Schwartz, Daniel P.; Agriculture Research Service, U.S. Department of Agriculture, Eastern Regional Research Center |
| Выпуск | 4 |
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