| Автор | Pellon, Gérard |
| Дата выпуска | 1992 |
| dc.description | AbstractThe laboratory preparation of chemically pure, <sup>3</sup>H-labelled β-propionylampicillin suitable for studies of the mode of action of β-lactam antibiotics has been investigated. The chemical purity of the labelled antibiotic is a crucial factor for the quantitative determination of penicillin-binding proteins. Ampicillin (D-α-aminobenzylpenicillin) was N-[<sup>3</sup>H]propionylated by N-succinimidyl [<sup>3</sup>H]propionate, the labelled reaction products were analyzed by thin-layer chromatography followed by fluorography. The synthesis and the purification of the resulting N-[<sup>3</sup>H]propionylampicillin have been optimized, the best conditions for the acylation reaction were at pH 8.25 for 2 h, giving both a high yield of the labelled antibiotic and minimal amounts of unwanted by-products. The reaction mixture was fractionated by a two-step procedure, the purified radioactive antibiotic did not contain any residual reactant, especially free ampicillin, or degradation products such as D-α.-[<sup>3</sup>H]propionamidobenzylpenicilloic acid. This improved procedure allows an easy, fast and rather inexpensive preparation of a good quality radiolabeled antibiotic for studies of penicillin-interacting proteins. |
| Формат | application.pdf |
| Издатель | Taylor & Francis Group |
| Копирайт | Copyright Taylor and Francis Group, LLC |
| Тема | labelled antibiotics |
| Тема | penicillin-binding proteins |
| Тема | N-[<sup>3</sup>H] porpionylampicillin |
| Название | An Easy Preparation of Chemically Pure, High-Activity Tritium-Labelled N-Propionylampicillin for the Analysis of Penicillin-Binding Proteins |
| Тип | research-article |
| DOI | 10.1080/00032719208017960 |
| Electronic ISSN | 1532-236X |
| Print ISSN | 0003-2719 |
| Журнал | Analytical Letters |
| Том | 25 |
| Первая страница | 2249 |
| Последняя страница | 2263 |
| Аффилиация | Pellon, Gérard; Laboratoire de Biochimie Microbienne (C. N. R. S., E. R. X. 0028), Université Claude Bernard-Lyon 1 |
| Выпуск | 12 |
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