| Автор | Das Gupta, S. |
| Автор | Ghosh, D. K. |
| Автор | Majumder, H. K. |
| Дата выпуска | 1991 |
| dc.description | SUMMARYDNA–DNA hybridization techniques have been found to be very useful in the classification and identification of Leishmania parasites. We report here the cloning of a mini-circle from Leishmania strain UR6 in a plasmid and subcloning of the mini-circle fragments in M13 mp9. Clone MLURk32, containing a 560 bp fragment of mini-circle, has been found to have unique specificity. Application of this specific probe in identifying different Leishmania isolates reveals that the probe reacted only with strains of post-kala-azar dermal leishmaniasis but not with strains or isolates of visceral leishmaniasis. |
| Формат | application.pdf |
| Издатель | Cambridge University Press |
| Копирайт | Copyright © Cambridge University Press 1991 |
| Тема | Leishmania spp. |
| Тема | mini-circle |
| Тема | hybridization |
| Тема | DNA probes |
| Название | A cloned kinetoplast DNA mini-circle fragment from a Leishmania spp. specific for post-kala-azar dermal leishmaniasis strains |
| Тип | research-article |
| DOI | 10.1017/S0031182000062478 |
| Electronic ISSN | 1469-8161 |
| Print ISSN | 0031-1820 |
| Журнал | Parasitology |
| Том | 102 |
| Первая страница | 187 |
| Последняя страница | 191 |
| Аффилиация | Das Gupta S.; Indian Institute of Chemical Biology |
| Аффилиация | Ghosh D. K.; Indian Institute of Chemical Biology |
| Аффилиация | Majumder H. K.; Indian Institute of Chemical Biology |
| Выпуск | 2 |
