ABSTRACTWe present the first record of Holocene and Pleistocene environmental change derived from the chemical and stable-isotope composition of a tropical cave guano sequence from Makangit Cave in northern Palawan (Philippines). The 180 cm sequence of guano, derived predominantly from insectivorous bats and birds, consists of two distinct units. An upper section of reddish-brown oxidised guano to 110 cm was deposited since the mid-Holocene while a lower section of black, reduced guano was deposited through the Last Glacial Maximum (LGM) to >30 000 BP. Carbon-isotope (δ¹³C) values in guano deposited during the LGM are as high as −13·5‰ indicating that a C₄-dominated grassland existed in the area around the cave at this time. Guano δ¹³C values of − 25‰ to − 28‰ suggest that this open vegetation was replaced by C₃-dominated closed tropical forest, similar to that of the present, by the mid-Holocene. The results suggest that the climate of northern Palawan was substantially drier at the LGM than is currently the case.