| Автор | Frederix, P.L.T.M. |
| Автор | Hoogenboom, B.W. |
| Автор | Fotiadis, D. |
| Автор | Müller, D.J. |
| Автор | Engel, A. |
| Дата выпуска | 2004 |
| dc.description | AbstractThe atomic force microscope (AFM) allows biomolecules to be observed and manipulated under native conditions. It produces images with an outstanding signal-to-noise ratio and addresses single molecules while the sample is in a buffer solution. Progress in sample preparation and instrumentation has led to topographs that reveal subnanometer details and the surface dynamics of biomolecules. Tethering single molecules between a support and a retracting AFM tip produces force–extension curves, giving information about the mechanical stability of secondary structural elements. For both imaging and force spectroscopy, the cantilever and its tip are critical:the mechanical properties of the cantilever dictate the force sensitivity and the scanning speed, whereas the tip shape determines the achievable lateral resolution. |
| Формат | application.pdf |
| Издатель | Cambridge University Press |
| Копирайт | Copyright © Materials Research Society 2004 |
| Тема | atomic force microscopy |
| Тема | biological molecules |
| Тема | imaging |
| Тема | native membranes |
| Тема | protein dynamics |
| Тема | single-molecule force spectroscopy. |
| Тема | Technical Feature |
| Название | Atomic Force Microscopy of Biological Samples |
| Тип | research-article |
| DOI | 10.1557/mrs2004.138 |
| Electronic ISSN | 1938-1425 |
| Print ISSN | 0883-7694 |
| Журнал | MRS Bulletin |
| Том | 29 |
| Первая страница | 449 |
| Последняя страница | 455 |
| Выпуск | 7 |
