A Bacillus subtilis Secretion Vector System Derived from the B. subtilis α-Amylase Promoter and Signal Sequence Region, and Secretion of Escherichia coli β-Lactamase by the Vector System<sup>1</sup>
OHMURA, Kazutaka; SHIROZA, Teruaki; NAKAMURA, Kouji; NAKAYAMA, Akira; YAMANE, Kunio; YODA, Koji; YAMASAKI, Makan; TAMURA, Gakuzo; OHMURA Kazutaka; Institute of Biological Sciences, University of Tsukuba; SHIROZA Teruaki; Institute of Biological Sciences, University of Tsukuba; NAKAMURA Kouji; Institute of Biological Sciences, University of Tsukuba; NAKAYAMA Akira; Institute of Biological Sciences, University of Tsukuba; YAMANE Kunio; Institute of Biological Sciences, University of Tsukuba; YODA Koji; Department of Agricultural Chemistry, The University of Tokyo; YAMASAKI Makan; Department of Agricultural Chemistry, The University of Tokyo; TAMURA Gakuzo; Department of Agricultural Chemistry, The University of Tokyo
Журнал:
Journal of Biochemistry
Дата:
1984
Аннотация:
A secretion vector system in Bacillus subtilis was constructed from the α-amylase promoter and signal sequence coding region of an α-amylase hyperproducing strain, B. subtilis NA64, and the major part of the plasmid pTUB4 which was derived from pUB110. When an Escherichia coli β-lactamase gene, lacking its own promoter and signal sequence coding region, was introduced into the secretion vector system, β-lactamase was expressed in B. subtilis. In addition, more than 95% of the enzyme synthesized was secreted into the culture medium via the secretion vector system. Secreted β-lactamase crossreacted with rabbit antiserum raised against the E. coli enzyme.
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