| Автор | OHMURA, Kazutaka |
| Автор | SHIROZA, Teruaki |
| Автор | NAKAMURA, Kouji |
| Автор | NAKAYAMA, Akira |
| Автор | YAMANE, Kunio |
| Автор | YODA, Koji |
| Автор | YAMASAKI, Makan |
| Автор | TAMURA, Gakuzo |
| Дата выпуска | 1984 |
| dc.description | A secretion vector system in Bacillus subtilis was constructed from the α-amylase promoter and signal sequence coding region of an α-amylase hyperproducing strain, B. subtilis NA64, and the major part of the plasmid pTUB4 which was derived from pUB110. When an Escherichia coli β-lactamase gene, lacking its own promoter and signal sequence coding region, was introduced into the secretion vector system, β-lactamase was expressed in B. subtilis. In addition, more than 95% of the enzyme synthesized was secreted into the culture medium via the secretion vector system. Secreted β-lactamase crossreacted with rabbit antiserum raised against the E. coli enzyme. |
| Формат | application.pdf |
| Издатель | Oxford University Press |
| Копирайт | © BY THE JOURNAL OF BIOCHEMISTRY |
| Тема | Articles |
| Название | A Bacillus subtilis Secretion Vector System Derived from the B. subtilis α-Amylase Promoter and Signal Sequence Region, and Secretion of Escherichia coli β-Lactamase by the Vector System<sup>1</sup> |
| Тип | research-article |
| Electronic ISSN | 1756-2651 |
| Print ISSN | 0021-924X |
| Журнал | Journal of Biochemistry |
| Том | 95 |
| Первая страница | 87 |
| Последняя страница | 93 |
| Аффилиация | OHMURA Kazutaka; Institute of Biological Sciences, University of Tsukuba |
| Аффилиация | SHIROZA Teruaki; Institute of Biological Sciences, University of Tsukuba |
| Аффилиация | NAKAMURA Kouji; Institute of Biological Sciences, University of Tsukuba |
| Аффилиация | NAKAYAMA Akira; Institute of Biological Sciences, University of Tsukuba |
| Аффилиация | YAMANE Kunio; Institute of Biological Sciences, University of Tsukuba |
| Аффилиация | YODA Koji; Department of Agricultural Chemistry, The University of Tokyo |
| Аффилиация | YAMASAKI Makan; Department of Agricultural Chemistry, The University of Tokyo |
| Аффилиация | TAMURA Gakuzo; Department of Agricultural Chemistry, The University of Tokyo |
| Выпуск | 1 |
