An improved indirect competitive Elisa for aflatoxin m<sub>1</sub> in milk powders using novel monoclonal antibodies
Okumura, Hiroki; Okimoto, Junko; Kishimoto, Seishi; Hasegawa, Akihiro; Kawamura, Osamu; Nakajima, Masahiro; Miyabe, Masaki; Ueno, Yoshio; Okumura, Hiroki; Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo; Okimoto, Junko; Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo; Kishimoto, Seishi; Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo; Hasegawa, Akihiro; Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo; Kawamura, Osamu; Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo; Research Institute for Biosciences, Science University of Tokyo; Nakajima, Masahiro; Food Department, Nagoya City Public Health Research Institute; Miyabe, Masaki; Food Department, Nagoya City Public Health Research Institute; Ueno, Yoshio; Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo; Research Institute for Biosciences, Science University of Tokyo
Журнал:
Food and Agricultural Immunology
Дата:
1993
Аннотация:
Among three newly prepared monoclonal anti‐aflatoxin M<sub>1</sub> antibodies, named AM.1, AM.2 and AM.3, both AM.1 and AM.3 possessed high specificity and sensitivity towards aflatoxin M<sub>1</sub>, while AM.2 exhibited lower specificity. Employing AM.3 and horseradish peroxidase‐labelled second antibody, an improved ELISA was developed with a detection limit of 1.0 pg ml<sup>‐1</sup> of aflatoxin M<sub>1</sub> in solution. The contents of aflatoxin M<sub>1</sub> in powdered milks suspended in water were assayed by the indirect ELISA. The detection limit was 5 pg g<sup>‐1</sup>dry weight, and no clean‐up procedures were required. The reliability of the present ELISA with monoclonal antibody AM.3 was confirmed with reference powdered milks for aflatoxin M<sub>1</sub>. A limited ELISA survey showed the presence of aflatoxin M<sub>1</sub> in commercial milk powders sampled in France, the US and Thailand at levels of 30–418 pg g<sup>‐1</sup>, and it was confirmed by an improved HPLC analysis.
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