| Автор | Okumura, Hiroki |
| Автор | Okimoto, Junko |
| Автор | Kishimoto, Seishi |
| Автор | Hasegawa, Akihiro |
| Автор | Kawamura, Osamu |
| Автор | Nakajima, Masahiro |
| Автор | Miyabe, Masaki |
| Автор | Ueno, Yoshio |
| Дата выпуска | 1993 |
| dc.description | Among three newly prepared monoclonal anti‐aflatoxin M<sub>1</sub> antibodies, named AM.1, AM.2 and AM.3, both AM.1 and AM.3 possessed high specificity and sensitivity towards aflatoxin M<sub>1</sub>, while AM.2 exhibited lower specificity. Employing AM.3 and horseradish peroxidase‐labelled second antibody, an improved ELISA was developed with a detection limit of 1.0 pg ml<sup>‐1</sup> of aflatoxin M<sub>1</sub> in solution. The contents of aflatoxin M<sub>1</sub> in powdered milks suspended in water were assayed by the indirect ELISA. The detection limit was 5 pg g<sup>‐1</sup>dry weight, and no clean‐up procedures were required. The reliability of the present ELISA with monoclonal antibody AM.3 was confirmed with reference powdered milks for aflatoxin M<sub>1</sub>. A limited ELISA survey showed the presence of aflatoxin M<sub>1</sub> in commercial milk powders sampled in France, the US and Thailand at levels of 30–418 pg g<sup>‐1</sup>, and it was confirmed by an improved HPLC analysis. |
| Формат | application.pdf |
| Издатель | Taylor & Francis Group |
| Копирайт | Copyright Taylor and Francis Group, LLC |
| Тема | Aflatoxin M<sub>1</sub> |
| Тема | ELISA |
| Тема | HPLC |
| Тема | monoclonal antibody |
| Тема | milk powder |
| Название | An improved indirect competitive Elisa for aflatoxin m<sub>1</sub> in milk powders using novel monoclonal antibodies |
| Тип | research-article |
| DOI | 10.1080/09540109309354786 |
| Electronic ISSN | 1465-3443 |
| Print ISSN | 0954-0105 |
| Журнал | Food and Agricultural Immunology |
| Том | 5 |
| Первая страница | 75 |
| Последняя страница | 84 |
| Аффилиация | Okumura, Hiroki; Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo |
| Аффилиация | Okimoto, Junko; Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo |
| Аффилиация | Kishimoto, Seishi; Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo |
| Аффилиация | Hasegawa, Akihiro; Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo |
| Аффилиация | Kawamura, Osamu; Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo; Research Institute for Biosciences, Science University of Tokyo |
| Аффилиация | Nakajima, Masahiro; Food Department, Nagoya City Public Health Research Institute |
| Аффилиация | Miyabe, Masaki; Food Department, Nagoya City Public Health Research Institute |
| Аффилиация | Ueno, Yoshio; Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo; Research Institute for Biosciences, Science University of Tokyo |
| Выпуск | 2 |
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