A highly specific detection method for diethylstilbestrol in bovine urine by radioimmunoassay following high performance liquid chromatography
Jansen, E. H. J. M.; van den Berg, R. H.; Van Blitterswijk, H.; Both‐Miedema, R.; Stephany, R. W.; Jansen, E. H. J. M.; Laboratory for Endocrinology, National Institute of Public Health and Environmental Hygiene; van den Berg, R. H.; Laboratory for Endocrinology, National Institute of Public Health and Environmental Hygiene; Van Blitterswijk, H.; Laboratory for Endocrinology, National Institute of Public Health and Environmental Hygiene; Both‐Miedema, R.; Laboratory for Endocrinology, National Institute of Public Health and Environmental Hygiene; Stephany, R. W.; Laboratory for Endocrinology, National Institute of Public Health and Environmental Hygiene
Журнал:
Food Additives and Contaminants
Дата:
1985
Аннотация:
A specific radioimmunoassay (RIA) for diethylstilbestrol (DES) is presented following purification of sample extracts by isocratic reversed phase high performance liquid chromatography. Applications are given of a forensic investigation for the control of DES in bovine urine. Specificity of HPLC‐RIA is compared with that of RIA's with other (Chromatographic) purification procedures and with gas chromatography‐mass spectrometry. Implications of the use of these techniques in practice and the use of various DES‐antisera are discussed.
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